Abruzzo M A, Mayer M, Jacobs P A
Cytogenet Cell Genet. 1985;39(4):275-8. doi: 10.1159/000132157.
It has been known for some time that there is an association between chronological aging and X-chromosome aneuploidy in peripheral blood lymphocyte cultures from females. In an attempt to elucidate the mechanism of X-chromosome aneuploidy in aging females, we used a BrdU late-labeling technique to determine the X-inactivation pattern in 45,X and 47,XXX lymphocytes of older women. In 50 of 58 X-aneuploid cells the inactive X chromosome was missing or extra. This implies that either the inactive X has a special propensity for mitotic errors or mitotic errors occur at random but subsequent selection is less stringent against cells with a missing or additional inactive X chromosome than against aneuploid cells involving the active X chromosome. Evidence is presented in favor of the former hypothesis.
一段时间以来，人们已经知道，在女性外周血淋巴细胞培养物中，按时间顺序的衰老与X染色体非整倍体之间存在关联。为了阐明衰老女性中X染色体非整倍体的机制，我们使用了BrdU晚期标记技术来确定老年女性45,X和47,XXX淋巴细胞中的X染色体失活模式。在58个X非整倍体细胞中的50个中，失活的X染色体缺失或多余。这意味着要么失活的X对有丝分裂错误有特殊倾向，要么有丝分裂错误随机发生，但随后对具有缺失或额外失活X染色体的细胞的选择比对涉及活性X染色体的非整倍体细胞的选择宽松。文中提供了支持前一种假设的证据。
