Davran Bulut Sena, Derebası Buse Nur, Günay Sevilay, Erden Yavuz, Celebioglu Hasan Ufuk, Ünal Mustafa Ümit
Department of Biotechnology, Faculty of Science, Bartin University, Bartin, Turkey.
Department of Biophysics, Faculty of Medicine, Gazi University, Ankara, Turkey.
Probiotics Antimicrob Proteins. 2025 Jun 20. doi: 10.1007/s12602-025-10631-1.
Probiotic bacteria are microorganisms that confer health benefits on the host, when administered in adequate amounts. Catechin, naturally found in nature and human diet, has strong antioxidant, antibacterial, and anticancer potentials. Phenolic compounds and probiotic bacteria are found together in gastrointestinal tract (GIT), and their potential synbiotic interactions are not fully understood. The present study aims to investigate the probiotic properties effects of catechin on (bacterial growth, auto-aggregation, co-aggregation) on well-known probiotic bacteria Lactobacillus acidophilus LA-5 (LA-5) and Lacticaseibacillus rhamnosus GG (LGG), and in vitro cytotoxic effects of the synbiotic combination of catechin and probiotic bacteria on colon cancer cells Caco-2. Results show that catechin contributed to bacterial growth of LA-5, auto-aggregation of LA-5, co-aggregation of LA-5 with Staphylococcus aureus. Catechin increased the adhesion potential of LA-5 and LGG by 6% and 5%, respectively. In DPPH scavenging activity, LGG grown with catechin exerted 30% more antioxidant potential compared the LGG. Moreover, LA-5 grown in the presence of catechin led to higher cell death of Caco-2 when compared to control while it did not influence the genotoxicity property of LA-5. However, catechin did not promote the cytotoxicity and genotoxicity properties of LGG. In conclusion, catechin enhanced many physiological properties of probiotic bacteria and thus, synbiotic relationship of catechin and probiotics may offer novel insights into the prevention/treatment of colon cancer.
益生菌是一类微生物，当给予足够数量时，能给宿主带来健康益处。儿茶素天然存在于自然界和人类饮食中，具有强大的抗氧化、抗菌和抗癌潜力。酚类化合物和益生菌在胃肠道中共同存在，它们潜在的合生元相互作用尚未完全了解。本研究旨在调查儿茶素对著名益生菌嗜酸乳杆菌LA - 5（LA - 5）和鼠李糖乳杆菌GG（LGG）的益生菌特性（细菌生长、自聚集、共聚集）的影响，以及儿茶素与益生菌的合生元组合对结肠癌细胞Caco - 2的体外细胞毒性作用。结果表明，儿茶素有助于LA - 5的细菌生长、LA - 5的自聚集以及LA - 5与金黄色葡萄球菌的共聚集。儿茶素分别使LA - 5和LGG的黏附潜力提高了6%和5%。在DPPH清除活性方面，与儿茶素一起生长的LGG相比LGG具有高出30%的抗氧化潜力。此外，与对照相比，在儿茶素存在下生长的LA - 5导致Caco - 2细胞死亡更高，同时它不影响LA - 5的遗传毒性特性。然而，儿茶素并未促进LGG的细胞毒性和遗传毒性特性。总之，儿茶素增强了益生菌的许多生理特性，因此，儿茶素与益生菌的合生元关系可能为结肠癌的预防/治疗提供新的见解。
