Sumi H, Seiki M, Morimoto N, Tsushima H, Maruyama M, Mihara H
Enzyme. 1985;33(3):122-7.
Plasma fibrinolysis in rats rose above the level of physiological fluctuations in a curve with two peaks at 1 and 6 h, following intraduodenal administration of high-molecular-weight urokinase (HMW-UK; MW 53,000; 124,000 IU/mg protein). Activation of plasma fibrinolysis was also confirmed with insolubilized enzyme (glass-coupled UK), but lacked the first activity peak. Plasma fibrinolytic enzyme isolated by affinity chromatography revealed strong fibrinolytic (1,120 IU/dl), pyro-Glu-Gly-Arg-pNA amidolytic (3,200 nmol/dl) and Glu-plasminogen activating (24.5 IU/dl) activities. Using specific UK antibody, it appeared that the first peak originated from the administered UK, while the second one derived from endogenous plasminogen activator. Dose response of UK was not observed, and the maximal effect was at about 5,000 IU/kg body weight.
十二指肠内给予高分子量尿激酶（HMW-UK；分子量53,000；124,000 IU/mg蛋白）后，大鼠血浆纤维蛋白溶解呈曲线上升，超过生理波动水平，在1小时和6小时出现两个峰值。用固定化酶（玻璃偶联尿激酶）也证实了血浆纤维蛋白溶解的激活，但没有第一个活性峰值。通过亲和层析分离的血浆纤维蛋白溶解酶显示出很强的纤维蛋白溶解活性（1,120 IU/dl）、焦谷氨酸-甘氨酸-精氨酸-对硝基苯胺酰胺水解活性（3,200 nmol/dl）和谷氨酸纤溶酶原激活活性（24.5 IU/dl）。使用特异性尿激酶抗体，似乎第一个峰值源于所给予的尿激酶，而第二个峰值来自内源性纤溶酶原激活剂。未观察到尿激酶的剂量反应，最大效应出现在约5,000 IU/kg体重时。
