Shi Xinrui, Yan Hao, Li Jingqi, Tang Yajie, Yang Songxin, Yao Yuanyuan, Wang Yueliang, Chen Hailan, Guo Longhua
Jiaxing Key Laboratory of Molecular Recognition and Sensing, College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing, 314001, China; College of Animal Science and Technology, Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Guangxi University, Nanning, Guangxi, 530005, China.
Jiaxing Key Laboratory of Molecular Recognition and Sensing, College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing, 314001, China.
Biosens Bioelectron. 2025 Nov 1;287:117699. doi: 10.1016/j.bios.2025.117699. Epub 2025 Jun 17.
Porcine epidemic diarrhea virus (PEDV) is the etiological agent of porcine epidemic diarrhea (PED), which is an acute and devastating enteric disease in suckling piglets and shows cross-species transmission ability between pigs and humans as well. The accurate and timely detection of PEDV infection is essential for the control and prevention of viral transmission and outbreaks. Here, a strong plasmon coupled gold nanoantennas have been developed by Ag induced gold nanoparticles (AuNPs) assembly for surface-enhanced Raman scattering (SERS) integrated lateral flow immunoassay (LFIA) toward rapid and ultrasensitive determination of PEDV. The nanoantennas own sub-nm interparticle gaps, where showing a 1.26 ×10-fold improvement of the electromagnetic Raman enhancement (|E|/|E|) compared with the AuNPs. The sub-nm gaps are a novel kind of closed SERS "hot spot", which can block large size molecule like antibody and BSA from entering gaps and replacing Raman dyes inside. The established gold nanoantennas based SERS-LFIA exhibited high sensitivity with limits of detection (LOD) as low as 1.0 × 10 and 1.0 TCID/mL according to naked eye observation and SERS detection, respectively. The SERS signal was linearly related to the PEDV concentration in the range of 1.0 × 10 to 1.0 × 10° TCID/mL, with a linear equation of y = 3560 x+1843 (R = 0.99916). The established assay shows excellent specificity, reproducibility, and stability. The recovery rate of standardly added clinical samples was 94.91 %-102.07 %. Therefore, the gold nanoantennas based SERS-LFIA have great potential for the rapid and sensitive determination of PEDV, which will facilitate the disease diagnosis and prevention.
猪流行性腹泻病毒（PEDV）是猪流行性腹泻（PED）的病原体，PED是一种发生于哺乳仔猪的急性、毁灭性肠道疾病，并且在猪和人之间也具有跨物种传播能力。准确及时地检测PEDV感染对于控制和预防病毒传播及疫情爆发至关重要。在此，通过银诱导金纳米颗粒（AuNPs）组装开发了一种强等离子体耦合金纳米天线，用于表面增强拉曼散射（SERS）集成侧向流动免疫分析（LFIA），以实现对PEDV的快速超灵敏测定。该纳米天线具有亚纳米级的粒子间间隙，与AuNPs相比，其电磁拉曼增强（|E|/|E|）提高了1.26×10倍。亚纳米级间隙是一种新型的封闭SERS“热点”，可阻止抗体和牛血清白蛋白等大分子进入间隙并取代内部的拉曼染料。所建立的基于金纳米天线的SERS-LFIA表现出高灵敏度，根据肉眼观察和SERS检测，检测限（LOD）分别低至1.0×10和1.0 TCID/mL。SERS信号在1.0×10至1.0×10°TCID/mL范围内与PEDV浓度呈线性相关，线性方程为y = 3560x + 1843（R = 0.99916）。所建立的检测方法具有出色的特异性、重现性和稳定性。标准添加临床样本的回收率为94.91%-102.07%。因此，基于金纳米天线的SERS-LFIA在快速灵敏测定PEDV方面具有巨大潜力，这将有助于疾病的诊断和预防。
