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宏基因组分析揭示了三种哺乳动物模型中肠道噬菌体的多样性。

Metagenomic analysis reveals gut phage diversity across three mammalian models.

作者信息

Yu Menghao, Chu Yunmeng, Wang Yongming, Mo Luofei, Tan Xin, Guo Shun, Yuan Shengjian, Ma Yingfei

机构信息

State Key Laboratory of Quantitative Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.

University of Chinese Academy of Sciences, Beijing, 100049, China.

出版信息

Microbiome. 2025 Jun 20;13(1):146. doi: 10.1186/s40168-025-02144-4.

DOI:10.1186/s40168-025-02144-4

PMID:40542420

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12180220/

Abstract

BACKGROUND

The gut virome plays a pivotal role in shaping the host's microbiota. However, gut viruses across different mammalian models, and their connections with the human gut microbiota remain largely unknown.

RESULTS

We identified 977 high-confidence species-level viral operational taxonomic units (vOTUs) in mice (hcMGV), 12,896 in pigs (hcPGV), and 1480 in cynomolgus macaques (hcCMGV) from metagenomes, respectively. Clustering these vOTUs at approximately genus level uncovered novel clades with high prevalence across animal guts (> = 60%). In particular, crAss-like phages and cas-harboring jumbophages were characterized. Comparative analysis revealed that hcCMGV had a closer relationship with hcPGV than hcMGV, despite the animal-specific characteristics, and that 55.88% hcCMGV had connections with the human microbiota.

CONCLUSIONS

Our findings shed light on the diversity of gut viruses across these three animals, contributing to future gut microbial studies using model animals. Video Abstract.

摘要

背景

肠道病毒组在塑造宿主微生物群方面起着关键作用。然而，不同哺乳动物模型中的肠道病毒及其与人类肠道微生物群的联系在很大程度上仍不清楚。

结果

我们分别从宏基因组中鉴定出小鼠中的977个高可信度物种水平病毒操作分类单元（vOTUs）（hcMGV）、猪中的12896个（hcPGV）和食蟹猴中的1480个（hcCMGV）。将这些vOTUs聚类到大约属水平，发现了在动物肠道中具有高流行率（≥60%）的新分支。特别是，对类crAss噬菌体和携带cas的巨型噬菌体进行了表征。比较分析表明，尽管具有动物特异性特征，但hcCMGV与hcPGV的关系比与hcMGV更密切，并且55.88%的hcCMGV与人类微生物群有联系。