Sheela Soumya, AlGhalban Fatma Mousa, Khalil Khalil Abdelrazek, Gopinath Vellore Kannan
Research Institute for Medical and Health Sciences, University of Sharjah, Sharjah, United Arab Emirates.
Department of Mechanical & Nuclear Engineering, College of Engineering, University of Sharjah, Sharjah, United Arab Emirates.
Clin Oral Investig. 2025 Jun 21;29(7):346. doi: 10.1007/s00784-025-06426-x.
To develop electrospun polycaprolactone (PCL) membranes incorporating Hydroxyapatite/Mineral Trioxide Aggregate (HAp/MTA) loaded with Resolvin D2 (RvD2) and evaluate its anti-inflammatory potential.
Electrospun membranes were fabricated by blending PCL with 10 wt.% HAp/10 wt.% MTA loaded with or without 0.1% RvD2 and were characterized using Field Emission Scanning Electron Microscopy (FE-SEM), energy-dispersive X-ray (EDX) spectroscopy, Raman spectroscopy, contact angle measurements, and RvD2 release.
The field emission scanning microscopy (FE-SEM) showed uniform nanofibers with an average fiber diameter ranging from 300 to 600 nm. Energy-dispersive X-ray (EDX) analysis and Raman spectroscopy confirmed the presence of HAp and MTA in the PCL membranes. The drug release profile displayed a rapid release of RvD2 initially, followed by a sustained release. XTT assay demonstrated the cytocompatibility of the membranes towards the THP-1-derived M1 macrophages and human dental pulp stem cells (hDPSCs) co-cultures activated with LPS. The FE-SEM studies proved the favorable cell adhesion behaviour although the membranes being hydrophobic. Cytokine levels in the culture supernatants retrieved from the co-culture treated membranes showed that, of all the membranes, RvD2 incorporated PCLHAp/MTA membranes demonstrated reduced IL-1β and TNF-α production.
RvD2 incorporated PCLHAp/MTA membranes demonstrated reduced IL-1β and TNF-α production asserting its anti-inflammotory potential.
This research highlights the immunomodulatory effect of RvD2-loaded PCLHAp/MTA electrospun membranes in reducing inflammation of the dental pulp. This novel method has the potential to transform the treatment of dental pulp inflammation resulting from caries or injury.
制备负载了消退素D2（RvD2）的羟基磷灰石/矿物三氧化物凝聚体（HAp/MTA）的静电纺聚己内酯（PCL）膜，并评估其抗炎潜力。
通过将PCL与负载或未负载0.1% RvD2的10 wt.% HAp/10 wt.% MTA混合来制备静电纺膜，并使用场发射扫描电子显微镜（FE-SEM）、能量色散X射线（EDX）光谱、拉曼光谱、接触角测量和RvD2释放进行表征。
场发射扫描显微镜（FE-SEM）显示出平均纤维直径在300至600纳米范围内的均匀纳米纤维。能量色散X射线（EDX）分析和拉曼光谱证实了PCL膜中存在HAp和MTA。药物释放曲线显示RvD2最初快速释放，随后持续释放。XTT试验证明了该膜对经脂多糖激活的THP-1衍生的M1巨噬细胞和人牙髓干细胞（hDPSCs）共培养物的细胞相容性。FE-SEM研究证明尽管膜具有疏水性，但具有良好的细胞粘附行为。从共培养处理过的膜中回收的培养上清液中的细胞因子水平表明，在所有膜中，负载RvD2的PCLHAp/MTA膜显示出IL-1β和TNF-α产生减少。
负载RvD2的PCLHAp/MTA膜显示出IL-1β和TNF-α产生减少，证明了其抗炎潜力。
本研究突出了负载RvD2的PCLHAp/MTA静电纺膜在减轻牙髓炎症方面的免疫调节作用。这种新方法有可能改变因龋齿或损伤引起的牙髓炎症的治疗方法。
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