炎症牙髓干细胞（IDPSCs）在聚己内酯（PCL）纳米纤维与羟基磷灰石混合体上的牙源性分化。

Odontogenic differentiation of inflamed dental pulp stem cells (IDPSCs) on polycaprolactone (PCL) nanofiber blended with hydroxyapatite.

机构信息

College of Dental Medicine, University of Sharjah, University City.

Sharjah Institute for Medical Research, University of Sharjah, University City.

出版信息

Dent Mater J. 2021 Mar 31;40(2):312-321. doi: 10.4012/dmj.2020-005. Epub 2020 Oct 15.

DOI:10.4012/dmj.2020-005

PMID:33055433

Abstract

The objective of this study was to develop electrospun polycaprolactone (PCL) membranes blended with hydroxyapatite (HA) and evaluate its potential in differentiating inflamed dental pulp stem/progenitor cells (IDPSCs) into odontoblasts. Electrospun nanofibrous membrane consisting of PCL blended with 10 wt% and 15 wt% of HA were fabricated and the characterization was done by Scanning electron microscopy (SEM), Fourier- transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and contact angle analysis. Cytocompatibility, cell adhesion and odontogenic differentiation ability of the membranes were assessed by MTT, Live/Dead, SEM/DAPI and qPCR studies. The mineral deposition ability of the membranes with IDPSCs was estimated by SEM-EDS. The SEM analysis revealed a nanofibrous texture with an average fiber diameter of 140 nm for PCL, 220 nm for PCL10%HA and 250 nm for PCL15%HA. Among the membranes tested, PCL10%HA favored positive cell attachments, upregulated expression of DSPP and ALP gene and higher Ca/P ratio compared to PCL and PCL15%HA.

摘要

本研究旨在制备聚己内酯（PCL）与羟基磷灰石（HA）共混的静电纺丝膜，并评估其在诱导牙髓干细胞/前体细胞（IDPSCs）向成牙本质细胞分化中的应用潜力。制备了包含 10wt%和 15wt%HA 的 PCL 共混物的静电纺丝纳米纤维膜，并通过扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）、X 射线衍射（XRD）和接触角分析进行了表征。通过 MTT、Live/Dead、SEM/DAPI 和 qPCR 研究评估了膜的细胞相容性、细胞黏附和成牙本质分化能力。通过 SEM-EDS 评估了 IDPSCs 与膜的矿化沉积能力。SEM 分析显示，PCL、PCL10%HA 和 PCL15%HA 的平均纤维直径分别为 140nm、220nm 和 250nm，呈现出纳米纤维结构。在测试的膜中，与 PCL 和 PCL15%HA 相比，PCL10%HA 更有利于细胞的黏附，上调 DSPP 和 ALP 基因的表达，并具有更高的 Ca/P 比。