建立模拟细胞可塑性和再生的基因工程小鼠肺类器官的方案。
Protocol for establishing genetically engineered murine lung organoids mimicking cell plasticity and regeneration.
作者信息
Kim Bongjun, Park Jae-Il
机构信息
Department of Experimental Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA; Department of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
Department of Experimental Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA; Genetics and Epigenetics Program, The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences, Houston, TX 77030, USA.
出版信息
STAR Protoc. 2025 Jun 20;6(3):103911. doi: 10.1016/j.xpro.2025.103911.
Lung endothelial cells (LuECs) play crucial roles in lung regeneration and support lung organoids (LOs). However, detailed guidelines for LO culture using LuECs have not been reported yet. Here, we present an efficient protocol for generating and culturing murine LOs that mimic regeneration. We describe steps for the isolation of LuECs and lung epithelial cells, followed by LO culture. We also describe how to establish genetically engineered LOs using viral transduction. For complete details on the use and execution of this protocol, please refer to Kim et al..
肺内皮细胞（LuECs）在肺再生中发挥着关键作用，并支持肺类器官（LOs）。然而，尚未有关于使用LuECs进行LO培养的详细指南报道。在此，我们提出了一种高效的方案，用于生成和培养模拟再生的小鼠LOs。我们描述了分离LuECs和肺上皮细胞的步骤，随后进行LO培养。我们还描述了如何使用病毒转导建立基因工程LOs。有关本方案使用和执行的完整详细信息，请参考Kim等人的研究。
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