Wang C, Baker H W, Jennings M G, Burger H G, Lutjen P
Fertil Steril. 1985 Oct;44(4):484-8. doi: 10.1016/s0015-0282(16)48916-8.
The immunobead test was used to study the immunoglobulin class of antibodies on sperm before and after penetration through a microcolumn of cervical mucus. Sixteen men with positive sperm antibodies (positive sperm immobilization test) and sperm that penetrated cervical mucus in prior tests were selected for study. However, at the time of study, sperm from seven subjects could not be recovered from the microcolumn. The nine subjects from whom motile sperm were obtained after passage through the column had better sperm mucus penetration tests, lower proportions of sperm binding to anti-IgA immunobeads, and higher proportions of sperm with tail-tip-only binding. Sperm recovered after penetration through the mucus microcolumn displayed a greatly reduced binding to anti-IgA immunobeads in all nine subjects, whereas similar reductions in anti-IgG binding occurred only in four subjects. These results confirm that IgA and sperm-head-directed antibodies are more important than IgG and sperm tail-tip-directed antibodies in impairing sperm penetration of cervical mucus.
免疫珠试验用于研究精子穿透宫颈黏液微柱前后抗体的免疫球蛋白类别。选择了16名精子抗体呈阳性（精子制动试验阳性）且在先前试验中精子能穿透宫颈黏液的男性进行研究。然而，在研究时，有7名受试者的精子无法从微柱中回收。在精子通过微柱后获得活动精子的9名受试者，其精子黏液穿透试验结果更好，与抗IgA免疫珠结合的精子比例更低，仅尾尖结合的精子比例更高。在所有9名受试者中，通过黏液微柱穿透后回收的精子与抗IgA免疫珠的结合显著减少，而抗IgG结合的类似减少仅发生在4名受试者中。这些结果证实，在损害精子穿透宫颈黏液方面，IgA和针对精子头部的抗体比IgG和针对精子尾尖的抗体更重要。
