Zhang Zhonghui, Chen Jie, Xiong Ziqi, Lin Weijie, Yang Bohao, Zong Zhiwei, Shen Bing, Luo Hanxi, Liu Chen
Department of Clinical Laboratory, Peking University People's Hospital, Beijing, PR China.
School of Basic Medical Sciences, Peking University Health Science Center, Beijing, PR China.
Immunol Lett. 2025 Dec;276:107054. doi: 10.1016/j.imlet.2025.107054. Epub 2025 Jun 20.
This study aims to analyse the expression of CD37 in different B cell subsets from human peripheral blood, exploring changes in CD37 expression in B cells of patients with primary Sjögren's syndrome (pSS) and assessing their clinical relevance.
A total of 32 pSS patients and age- and sex-matched healthy controls were included. Peripheral blood mononuclear cells (PBMCs) were isolated, and B cells were classified into five subsets using flow cytometry based on CD24 and CD38 expression. CD37 expression was evaluated, and functional differences between CD37+ and CD37- B cells were compared. Peripheral lymphocytes were stimulated with LPS to observe changes in CD37 expression. The expression of CD37 and related B cell subsets was also compared between pSS patients and healthy controls, followed by ROC curve analysis.
CD37 was highly expressed in early-stage B cells, with a significant decrease in effector cells. CD37+ B cells exhibited higher proportions of CD40+, HLA-DR+, and IL-10+ cells, indicating enhanced antigen-presenting and regulatory capabilities. Post-LPS stimulation, CD37 expression significantly declined in pSS patients compared to healthy controls, suggesting increased sensitivity to differentiation into plasma cells. Additionally, CD37-related B cell subsets in pSS patients showed strong correlations with autoantibodies and immunological markers. ROC analysis revealed substantial areas under the curve, confirming the diagnostic potential of these subsets.
The expression of CD37 in B cell subsets is variable, indicating a potential regulatory role in B cell function and offering auxiliary diagnostic value in pSS.
本研究旨在分析CD37在人外周血不同B细胞亚群中的表达，探讨原发性干燥综合征（pSS）患者B细胞中CD37表达的变化，并评估其临床相关性。
共纳入32例pSS患者及年龄和性别匹配的健康对照。分离外周血单个核细胞（PBMC），基于CD24和CD38表达，采用流式细胞术将B细胞分为五个亚群。评估CD37表达，并比较CD37+和CD37- B细胞之间的功能差异。用脂多糖刺激外周淋巴细胞，观察CD37表达的变化。还比较了pSS患者和健康对照之间CD37及相关B细胞亚群的表达，随后进行ROC曲线分析。
CD37在早期B细胞中高表达，在效应细胞中显著降低。CD37+ B细胞中CD40+、HLA-DR+和IL-10+细胞比例较高，表明其抗原呈递和调节能力增强。脂多糖刺激后，与健康对照相比，pSS患者的CD37表达显著下降，提示其向浆细胞分化的敏感性增加。此外，pSS患者中与CD37相关的B细胞亚群与自身抗体和免疫标志物密切相关。ROC分析显示曲线下面积较大，证实了这些亚群的诊断潜力。
CD37在B细胞亚群中的表达存在差异，表明其在B细胞功能中可能具有调节作用，并在pSS中具有辅助诊断价值。
