白花蛇舌草多糖微波辅助提取工艺优化及其对理化性质、抗氧化和降血糖能力的影响：一级结构-活性关系
Optimization of microwave-assisted extraction for Herba Patriniae polysaccharide and its impact on physicochemical properties, antioxidant and hypoglycemic capacities: Primary structure-activity relationship.
作者信息
Hui Heping, Mao Haoji, Wei Jing, Xue Xiaodong, Cheng Min
机构信息
College of Biology Pharmacy and Food Engineering, Shangluo University, Shangluo, Shaanxi 726000, China.
College of Biology Pharmacy and Food Engineering, Shangluo University, Shangluo, Shaanxi 726000, China.
出版信息
Int J Biol Macromol. 2025 Aug;319(Pt 1):145396. doi: 10.1016/j.ijbiomac.2025.145396. Epub 2025 Jun 19.
This study optimized microwave-assisted extraction (MAE) of Herba Patriniae polysaccharide (HPP) and systematically compared its yield, properties and bioactivities with hot water extraction (HWE) and ultrasonic-assisted cellulase extraction (UACE). MAE (43.92 min, 437.31 W, liquid-to-material ratio 17.68: 1) enhanced HPP yields by 487.85 % and 6.13 % over HWE and UACE, respectively, while improving key physicochemical properties, including increased total sugar (112-116 %), uronic acids (>368 %), and HWE-like monosaccharide composition but elevated galactose (58.54 %), arabinose (54.49 %), and galacturonic acid content (704.76 %), alongside diminished particle size (1216.74 nm) and molecular weight (600 kDa). Bioactivity assays showed MAE-derived HPP' superior antioxidant capacity (18-63 % higher DPPH/hydroxyl radical scavenging) and enzyme inhibition (6-50 % greater α-glucosidase and α-amylase activity). Oxidative stress and insulin resistance HepG2 revealed MAE boosted antioxidant enzyme activities (SOD: 1.81-fold vs model, 1.02-1.08-fold vs HWE/UACE; CAT: 3.01-fold vs model, 1.06-1.43-fold vs HWE/UACE; GST-Px: 1.57-fold vs model, 1.03-1.49-fold vs HWE/UACE) and reduced lipid peroxidation (decreased 166 % MDA vs model, 12.5-25 % vs HWE/UACE). Hypoglycemic effects involved upregulated hexokinase (1.23-fold vs model, 1.03-1.08-fold vs HWE/UACE) and pyruvate kinase (1.13-fold vs model, 1.04-1.08-fold vs HWE/UACE), with suppressed Glucose-6-phosphatase (decreased by 12.5 % vs model, 5.76-7.55 % vs HWE/UACE). MAE is a promising technique for preparation HPP with desirable bioactivities.
本研究优化了微波辅助提取（MAE）白花败酱多糖（HPP）的工艺，并将其产率、性质和生物活性与热水提取（HWE）及超声辅助纤维素酶提取（UACE）进行了系统比较。MAE（43.92分钟，437.31瓦，料液比17.68:1）使HPP的产率分别比HWE和UACE提高了487.85%和6.13%，同时改善了关键理化性质，包括总糖含量增加（112 - 116%）、糖醛酸含量增加（>368%），单糖组成与HWE类似，但半乳糖含量升高（58.54%）、阿拉伯糖含量升高（54.49%）以及半乳糖醛酸含量升高（704.76%），同时粒径减小（1216.74纳米）、分子量降低（约600 kDa）。生物活性测定表明，MAE提取的HPP具有更强的抗氧化能力（DPPH/羟基自由基清除能力提高18 - 63%）和酶抑制能力（α - 葡萄糖苷酶和α - 淀粉酶活性提高6 - 50%）。在氧化应激和胰岛素抵抗的HepG2细胞中，MAE提取的HPP提高了抗氧化酶活性（超氧化物歧化酶：与模型组相比提高1.81倍，与HWE/UACE相比提高1.02 - 1.08倍；过氧化氢酶：与模型组相比提高3.01倍，与HWE/UACE相比提高1.06 - 1.43倍；谷胱甘肽过氧化物酶：与模型组相比提高1.57倍，与HWE/UACE相比提高1.03 - 1.49倍），并降低了脂质过氧化水平（丙二醛含量与模型组相比降低166%，与HWE/UACE相比降低12.5 - 25%）。降血糖作用涉及己糖激酶上调（与模型组相比提高1.23倍，与HWE/UACE相比提高1.03 - 1.08倍）和丙酮酸激酶上调（与模型组相比提高1.13倍，与HWE/UACE相比提高1.04 - 1.08倍），同时葡萄糖 - 6 - 磷酸酶受到抑制（与模型组相比降低12.5%，与HWE/UACE相比降低5.76 - 7.55%）。MAE是一种制备具有理想生物活性的HPP的有前景的技术。
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