Yang Caining, Zhang Yupei, Yang Congcong, Chen Jinyin, Yang Xiaorong, Chen Chuying, Kai Wenbin, Zeng Jiaoke, Chen Ming, Gan Zengyu
Jiangxi Provincial Key Laboratory for Postharvest Storage and Preservation of Fruits & Vegetables, College of Agronomy, Jiangxi Agricultural University, Nanchang, 330045, China.
Zhangshu City Farming and Animal Husbandry Breeding Center, Yichun, 331299, China.
Plant Physiol Biochem. 2025 Oct;227:110182. doi: 10.1016/j.plaphy.2025.110182. Epub 2025 Jun 19.
The content of reactive oxygen species (ROS) was closely associated with the postharvest quality and softening speed in kiwifruit. Here, the effects of blue light on postharvest softening and ROS metabolism of kiwifruit were studied. The results demonstrated that blue light with a wavelength of 420 nm resulted in a delay in the reduction of fruit firmness and accumulation of total soluble solids by augmenting 1,1- diphenyl-2-picrylhydrazyl scavenging rate and reducing the levels of malondialdehyde, hydrogen peroxide, and superoxide anion. Blue light concurrently enhanced the activity of superoxide dismutase (SOD) and catalase (CAT), as well as upregulated the expression levels of associated genes. Additionally, kiwifruit treated with blue light exhibited significantly elevated levels of ascorbic acid (AsA) and glutathione (GSH), along with reduced levels of oxidized AsA (DHA) and oxidized GSH (GSSG), in comparison to the control group. Also, blue light enhanced the activity of ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR), mono-DHAR (MDHAR), and GSH reductase (GR) enzymes in the AsA-GSH cycle, while it decreased the activity of AsA oxidase (AO) enzymes. Correspondingly, blue light induced the expression of genes related to AsA biosynthesis and the AsA-GSH cycle, while inhibiting the expression of the AcAO genes. Altogether, the findings of our study indicate that blue light effectively enhanced the ROS scavenging capacity of kiwifruit and preserved a high level of AsA content, then retarded its postharvest ripening and softening.
活性氧（ROS）的含量与猕猴桃采后的品质及软化速度密切相关。在此，研究了蓝光对猕猴桃采后软化及ROS代谢的影响。结果表明，波长为420 nm的蓝光通过提高1,1-二苯基-2-苦基肼清除率和降低丙二醛、过氧化氢和超氧阴离子的水平，延缓了果实硬度的降低和总可溶性固形物的积累。蓝光同时增强了超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的活性，并上调了相关基因的表达水平。此外，与对照组相比，经蓝光处理的猕猴桃抗坏血酸（AsA）和谷胱甘肽（GSH）水平显著升高，氧化型AsA（DHA）和氧化型GSH（GSSG）水平降低。此外，蓝光增强了AsA-GSH循环中抗坏血酸过氧化物酶（APX）、脱氢抗坏血酸还原酶（DHAR）、单脱氢抗坏血酸还原酶（MDHAR）和谷胱甘肽还原酶（GR）的活性，同时降低了AsA氧化酶（AO）的活性。相应地，蓝光诱导了与AsA生物合成和AsA-GSH循环相关基因的表达，同时抑制了AcAO基因的表达。总之，我们的研究结果表明，蓝光有效地增强了猕猴桃的ROS清除能力，保持了较高水平的AsA含量，从而延缓了其采后成熟和软化。
