花粉蛋白B在哮喘中的保护作用：PPAR-γ介导的炎症途径调节及花生四烯酸代谢

Protective effects of pollenin B in asthma: PPAR-γ-mediated regulation of inflammatory pathways and arachidonic acid metabolism.

作者信息

Wu Yuanyuan, Zeng Mengnan, Jiao Xinmian, Ma Xinyi, Wang Haoyu, Chen Yanling, Zheng Xiaoke, Feng Weisheng

机构信息

College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China; Engineering and Technology Center for Chinese Medicine Development of Henan Province, Zhengzhou 450046, China.

College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China; Engineering and Technology Center for Chinese Medicine Development of Henan Province, Zhengzhou 450046, China; Co-construction Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases by Henan & Education Ministry of China, Henan University of Chinese Medicine, Zhengzhou 450046, China.

出版信息

Phytomedicine. 2025 Sep;145:156975. doi: 10.1016/j.phymed.2025.156975. Epub 2025 Jun 11.

DOI:10.1016/j.phymed.2025.156975

PMID:40544731

Abstract

BACKGROUND

Asthma poses a significant global health challenge, characterized by increasing prevalence and treatment complexities. Although Ephedrae Herba has long been used in asthma therapy, its pharmacological basis and mechanism of action remain unclear.

PURPOSE

This study aimed to clarify the protective effects of pollenin B (PolB), a compound extracted from Ephedrae Herba, in asthmatic mice and to thoroughly explore its possible action pathways and molecular mechanisms. This study supplements pharmacological research on the anti-asthmatic effects of Ephedrae Herba and provides more information for the development of novel and highly effective drugs for the treatment of asthma.

METHODS

An ovalbumin (OVA)-induced asthma model was used to evaluate the effects of PolB on asthma symptoms and lung histopathology. Furthermore, cytokine profiles in the serum and bronchoalveolar lavage fluid (BALF), immune cell populations, and serum metabolomics data were analyzed to identify the pathways involved in PolB activity. PPAR-γ was identified as a key target, and its interaction with PolB was confirmed by surface plasmon resonance (SPR) analysis. A PPAR-γ inhibitor (T0070907) was administered in vivo, and combined PPAR-γ agonist (rosiglitazone)/inhibitor (T0070907) assays were conducted in vitro to validate the mechanisms involved.

RESULTS

PolB improved asthma symptoms, modulated cytokine levels in the serum and BALF, modulated immune cells, and influenced arachidonic acid metabolism. Further mechanistic exploration indicated that PolB mitigated airway inflammation by regulating arachidonic acid metabolism and the JAK-STAT pathway via PPAR-γ.

CONCLUSION

PolB exerts anti-asthmatic effects via PPAR-γ-mediated regulation of key pathways. These findings not only enrich modern pharmacological research on the anti-asthmatic properties of Ephedrae Herba but also deepen our understanding of asthma pathogenesis, thus providing new insights into the optimization of asthma prevention and treatment.

摘要

背景

哮喘是一项重大的全球健康挑战，其特点是患病率不断上升且治疗愈发复杂。虽然麻黄长期以来一直用于哮喘治疗，但其药理基础和作用机制仍不清楚。

目的

本研究旨在阐明从麻黄中提取的化合物花粉in B（PolB）对哮喘小鼠的保护作用，并深入探讨其可能的作用途径和分子机制。本研究补充了麻黄抗哮喘作用的药理学研究，并为开发治疗哮喘的新型高效药物提供了更多信息。

方法

采用卵清蛋白（OVA）诱导的哮喘模型来评估PolB对哮喘症状和肺组织病理学的影响。此外，分析血清和支气管肺泡灌洗液（BALF）中的细胞因子谱、免疫细胞群体以及血清代谢组学数据，以确定参与PolB活性的途径。PPAR-γ被确定为关键靶点，并通过表面等离子体共振（SPR）分析证实其与PolB的相互作用。在体内给予PPAR-γ抑制剂（T0070907），并在体外进行联合PPAR-γ激动剂（罗格列酮）/抑制剂（T0070907）试验以验证其中涉及的机制。