Hwang Seo Hyun, Noh Jun Su, Maung Chaw Ei Htwe, Kim Kil Yong
Department of Agricultural Chemistry, Environmentally-Friendly Agricultural Research Center, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Republic of Korea.
Department of Agricultural and Biological Chemistry, Environmentally-Friendly Agricultural Research Center, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Republic of Korea.
Microb Pathog. 2025 Sep;206:107829. doi: 10.1016/j.micpath.2025.107829. Epub 2025 Jun 20.
Southern blight, caused by Sclerotium rolfsii, is a highly destructive soilborne fungal disease that severely impacts peanut production worldwide. The rapid spread and aggressive nature of this pathogen, coupled with its ability to produce sclerotia that can persist in soil for extended periods, make it a significant threat to global agriculture. This study explored the biocontrol potential of volatile organic compounds (VOCs) produced by Bacillus amyloliquefaciens HK17 as an alternative approach to managing southern blight in peanut. VOCs produced by strain HK17 in two different growth media-tryptic soy broth medium (TSB-HK17) and sterile compost-amended soil (SCS-HK17)-effectively inhibited the mycelial growth and sclerotia germination of S. rolfsii and disrupted the synthesis of oxalic acid, a key virulence factor of S. rolfsii. They also caused a significant reduction in the activity of plant cell wall-degrading enzymes associated with fungal pathogenicity. Gas chromatography-mass spectrometry analysis showed the VOCs belonged to several classes of compounds, including alcohols, amines, and sulfur-containing compounds, with amines being particularly dominant. Notably, 5-nonylamine was the most effective in reducing the growth of mycelia from both mycelial plugs and germinated sclerotia. Treatment with TSB-HK17- and SCS-HK17-produced VOCs significantly controlled S. rolfsii invasion in peanut plants compared to untreated controls. These results indicate that VOCs produced by B. amyloliquefaciens HK17 could be utilized as promising, environmentally friendly agents for the effective management of southern blight in peanut and other crops, offering a sustainable alternative to traditional chemical pesticides.
由齐整小核菌引起的白绢病是一种极具破坏性的土传真菌病害，严重影响全球花生生产。这种病原菌传播迅速且具有侵袭性，再加上它能产生可在土壤中长期存活的菌核，使其成为全球农业的重大威胁。本研究探索了解淀粉芽孢杆菌HK17产生的挥发性有机化合物（VOCs）作为防治花生白绢病的替代方法的生物防治潜力。菌株HK17在两种不同生长培养基——胰蛋白胨大豆肉汤培养基（TSB-HK17）和无菌堆肥改良土壤（SCS-HK17）中产生的VOCs有效抑制了齐整小核菌的菌丝生长和菌核萌发，并破坏了齐整小核菌关键致病因子草酸的合成。它们还显著降低了与真菌致病性相关的植物细胞壁降解酶的活性。气相色谱-质谱分析表明，这些VOCs属于几类化合物，包括醇类、胺类和含硫化合物，其中胺类尤为占主导。值得注意的是，5-壬胺在抑制菌丝块和萌发菌核的菌丝生长方面最为有效。与未处理的对照相比，用TSB-HK17和SCS-HK17产生的VOCs处理显著控制了花生植株中齐整小核菌的侵染。这些结果表明，解淀粉芽孢杆菌HK17产生的VOCs可作为有前景的、环境友好型药剂用于有效防治花生和其他作物的白绢病，为传统化学农药提供了一种可持续的替代方案。
