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豚鼠结肠带平滑肌与汞离子结合的分析

Analysis of mercury ions binding to the smooth muscle of guinea-pig taenia coli.

作者信息

Nasu T, Gyobu K, Ishida Y

出版信息

Gen Pharmacol. 1985;16(5):513-5. doi: 10.1016/0306-3623(85)90014-x.

DOI:10.1016/0306-3623(85)90014-x
PMID:4054575
Abstract

Mercury uptake to taenia coli increased with an increase of the Hg2+ concentration (0.05-5 mmol/l). The Scatchard plots of mercury uptake showed that two qualitative Hg2+ binding sites (high and low affinity sites) exist in taenia coli. The Scatchard analysis of Hg2+ binding at 4 degrees C indicated a single class of binding site (high affinity binding site only). These results indicate that Hg2+ binds to high affinity binding sites of cell membrane and Hg2+ of low affinity binding sites may reflect intracellular accumulation in taenia coli.

摘要

随着Hg2+浓度(0.05 - 5 mmol/l)的增加,结肠带对汞的摄取量增加。汞摄取的Scatchard图表明,结肠带中存在两种性质的Hg2+结合位点(高亲和力和低亲和力位点)。4℃下Hg2+结合的Scatchard分析表明存在一类结合位点(仅高亲和力结合位点)。这些结果表明,Hg2+与细胞膜的高亲和力结合位点结合,低亲和力结合位点的Hg2+可能反映了结肠带中的细胞内积累。

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