Hoyer H E
Graefes Arch Clin Exp Ophthalmol. 1985;223(4):169-73. doi: 10.1007/BF02174055.
The influence of a calcium-enriched solution on pig lens cell structure was studied in vitro. The morphological changes which occurred 20 min or 2 h after bathing in the solution were studied by transmission electron microscopy. After 20-min incubation, small intercellular vacuoles containing membranous and amorphous substance occurred mainly in the epithelial cells. The cytoplasm of the cortical fibers had already been dissolved at this stage. Here the membrane was disrupted and the extracellular spaces widened. After 2-h incubation, the epithelial cells were compressed by adjacent swollen fibers. However, vacuolization in the epithelium had decreased at this stage. At the cortical fiber zone, vacuolar formation had increased extensively. Vacuolization was mainly observed in the intercellular spaces between the intact gap junctions. After 2 h, the main alterations were membrane disruptions and advancing dissolution of the cytoplasm. Deeper cortical fibers were swollen but showed no extracellular vacuoles.
在体外研究了富钙溶液对猪晶状体细胞结构的影响。通过透射电子显微镜研究了在该溶液中浸泡20分钟或2小时后发生的形态学变化。孵育20分钟后，主要在上皮细胞中出现含有膜状和无定形物质的小细胞间空泡。此时皮质纤维的细胞质已经溶解。此处膜被破坏，细胞外间隙增宽。孵育2小时后，上皮细胞被相邻肿胀的纤维挤压。然而，此时上皮细胞中的空泡化已经减少。在皮质纤维区，空泡形成大量增加。空泡化主要见于完整缝隙连接之间的细胞间隙。2小时后，主要变化是膜破坏和细胞质进一步溶解。更深层的皮质纤维肿胀但未出现细胞外空泡。
