Dubey J P, Streitel R H, Stromberg P C, Toussant M J
J Parasitol. 1977 Jun;63(3):443-7.
Hearts, diaphragms, esophagi, and spinal cords from 266 horses were obtained at slaughter in Creston, Ohio. Tissues were examined microscopically for Sarcocystis in sections, digested in trypsin to obtain bradyzoites, and fed to 10 dogs and 10 cats. Intramuscular cysts were found in selections of two hearts from 57 horses and four esophagi from 107 horses. The cysts were up to 900 micron long and up to 70 micron wide. The cyst wall was 1 to 2 micron thick and cross-striated. The enclosed bradyzoites were banana-shaped, 15 to 20 by 20 to 3 micron, and contained several PAS-positive granules. Bradyzoites were found in trypsin digests of seven of 57 (13%) equine tissues (heart, diaphragm, esophagus but not spinal cord) in one experiment and 10 of 47 (21%) esophagi, eight of 47 (17%) diaphragms but none of 47 hearts and spinal cords in another experiment. All of 10 dogs shed sporulated sporocysts or oocysts in feces 12 to 15 days (12 in one, 13 in eight, and 15 days in one) after digesting tissues from 169 horses. The sporocysts were 11 to 13 (12.0 +/- 0.5) by 7 to 8.5 (7.9 +/- 0.5) micron. In histologic sections of canine small intestine the sporocysts were located in the lamina propria near the tips of the villi. The 10 cats fed tissues from 266 horses did not shed Sarcocystis. A new name, S. fayeri, is proposed for this organism. Sarcocystis fayeri sporocysts (12 by 8 micron) are shorter than those of S. betrami (15 by 10 micron), the other species of Sarcocystis from the horse. The prepatent period is 12 to 15 days for S. fayeri and 8 days for S. bertrami (synonym S. equicanis Rommel and Geisel 1975).
从俄亥俄州克雷斯顿屠宰的266匹马身上获取心脏、横膈膜、食管和脊髓。对组织切片进行显微镜检查以寻找肉孢子虫，用胰蛋白酶消化组织以获取缓殖子，并将其喂食给10只狗和10只猫。在57匹马的两颗心脏样本以及107匹马的四条食管中发现了肌内囊肿。囊肿最长可达900微米，最宽可达70微米。囊肿壁厚1至2微米，有横纹。包囊内的缓殖子呈香蕉形，大小为15至20×20至3微米，含有数个过碘酸雪夫反应阳性颗粒。在一项实验中，57个马组织样本（心脏、横膈膜、食管但不包括脊髓）中的7个（13%）经胰蛋白酶消化后发现了缓殖子；在另一项实验中，47个食管样本中的10个（21%）、47个横膈膜样本中的8个（17%）发现了缓殖子，但47个心脏和脊髓样本中均未发现。10只狗在消化169匹马的组织后12至15天（1只狗在12天、8只狗在13天、1只狗在15天）粪便中均排出了孢子化的孢子囊或卵囊。孢子囊大小为11至13（12.0±0.5）×7至8.5（7.9±0.5）微米。在犬小肠组织切片中，孢子囊位于绒毛尖端附近的固有层。喂食266匹马组织的10只猫未排出肉孢子虫。为此种生物提出了一个新名称，即费氏肉孢子虫。费氏肉孢子虫的孢子囊（12×8微米）比马的另一种肉孢子虫——贝氏肉孢子虫（15×10微米）的孢子囊短。费氏肉孢子虫的潜隐期为12至15天，贝氏肉孢子虫（同义词为1975年的马肉孢子虫Rommel和Geisel）的潜隐期为8天。
