Dong Lijuan, Geng Boyang, Xu Yuting, Yu Wei, Yang Li-E, Peng Deli
Faculty of Geography, Yunnan Normal University, Kunming, Yunnan, China.
Yunnan Provincial Key Laboratory of Plateau Geographic Process and Environmental Change, Yunnan Normal University, Kunming, Yunnan, China.
PeerJ. 2025 Jun 18;13:e19559. doi: 10.7717/peerj.19559. eCollection 2025.
Seed dormancy and the requirements for germination following dormancy release are critical factors influencing the success of seedling establishment. This study examined six plant species from the dry-warm valley region of the Jinshajiang River in southwestern China, investigating their seed dormancy types and germination characteristics. Initially, germination tests were conducted using freshly matured seeds at alternating temperatures of 25/15 and 15/5 °C under light conditions. Subsequently, after dry after-ripening (DAR), germination was retested. Additionally, dried seeds were incubated under a range of constant temperatures (5-37 °C) under light conditions. The effects of darkness and GA on seed germination were evaluated at alternating temperatures of 25/15 and 15/5 °C. Cardinal temperatures and thermal time requirements for 50% final germination ( ) were determined. The increase in final germination following seed coat scarification indicated that seeds exhibited physical dormancy at dispersal. Treatment with DAR and/or GA effectively alleviated dormancy in the other five species (, , , , ), particularly at the cooler temperature regime of 15/5 °C, suggesting that these species possess non-deep physiological dormancy. Once dormancy is alleviated, seeds of all six plant species can germinate across a broad temperature spectrum, and the temperature window ( - ) for germination is much wider than the actual germination range. Alternating temperatures did not significantly enhance germination rates compared to constant temperatures, except for . Seeds of exhibited a strict light requirement for germination at alternating temperatures of 25/15 and 15/5 °C, whereas the other five plant species germinated effectively in darkness at the warmer alternating temperature of 25/15 °C. Thus, our hypothesis that dormancy and germination traits restrict germination to the summer (rainy season) is supported. This ensures that seedlings can establish themselves once soil moisture and temperature conditions become favorable. This research offers a valuable scientific reference for vegetation restoration efforts in dry-warm valley regions.
种子休眠以及休眠解除后的萌发条件是影响幼苗建成成功与否的关键因素。本研究对中国西南部金沙江干热河谷地区的6种植物进行了考察,研究了它们的种子休眠类型和萌发特性。首先,使用刚成熟的种子在光照条件下25/15和15/5℃变温条件下进行萌发试验。随后,进行干后熟处理(DAR)后,再次进行萌发测试。此外,将干燥种子在光照条件下一系列恒温(5 - 37℃)条件下培养。在25/15和15/5℃变温条件下评估黑暗和赤霉素对种子萌发的影响。确定了最终萌发率达到50%( )时的基点温度和热时间需求。种皮划破后最终萌发率的增加表明 种子在散布时表现出物理休眠。DAR和/或赤霉素处理有效地缓解了其他5种植物( 、 、 、 、 )的休眠,特别是在15/5℃较凉爽的温度条件下,这表明这些物种具有非深度生理休眠。一旦休眠解除,所有6种植物的种子都能在较宽的温度范围内萌发,并且萌发的温度窗口( - )比实际萌发范围宽得多。与恒温相比,变温并未显著提高萌发率, 除外。 的种子在25/15和15/5℃变温条件下萌发对光照有严格要求,而其他5种植物在25/15℃较温暖的变温条件下能在黑暗中有效萌发。因此,我们关于休眠和萌发特性将萌发限制在夏季(雨季)的假设得到了支持。这确保了一旦土壤湿度和温度条件适宜,幼苗就能成功建成。本研究为干热河谷地区的植被恢复工作提供了有价值的科学参考。