Adss Ibrahim A, R Bayoumi Shokry, Heikal Hesham M, Sobhy Sherien E, Saleh Ahmed A, Hafez Elsayed E
Division of Genetics, Faculty of Agriculture, Damanhour University, Al-Beheira, Egypt.
Department of Botany, Faculty of Science, Damanhour University, Al-Beheira,, Egypt.
Mol Biol Rep. 2025 Jun 23;52(1):625. doi: 10.1007/s11033-025-10728-4.
The interaction of various treatments such as abscisic acid (ABA), polyamines (PS), and biological control with Trichoderma against Meloidogyne incognita was evaluated to assess their impact on pathological indices, growth metrics, and defense mechanisms in tomato cultivars.
The study assessed Meloidogyne incognita root tomato disease (number of galls and egg masses) and growth parameters (shoot, root system, and fruit weight) in two tomato cultivars (1077 and Dania 85), along with the activities of defense enzymes; peroxidase (POD), polyphenol oxidase (PPO) and expression of defense genes (POD, PPO and PR2). Specifically, the study evaluated abscisic acid (ABA; 10 µM), potassium silicate (5 g/L), and biological Trichoderma harzianum (10 CFU/ml) treatments under greenhouse conditions 45 days post-inoculation. pathological indices, including the number of galls and egg masses, were measured, along with defense enzyme activities and gene expression levels.
Among the treatments applied, the 1077 cultivar demonstrated the highest resistance, exhibiting the lowest number of galls and egg masses. All treatments (ABA, PS, and Trichoderma) significantly increased growth parameters compared to the nematode-infected untreated control. Specifically, PS treatment showed the strongest enhancement in shoot fresh weight across both cultivars (1077 and Dania 85). Enzyme activities of PPO and POD, along with the gene expressions of POD, PPO and PR2, were elevated across all treatments. PS was the most effective treatment, followed by ABA and Trichoderma, significantly boosting both enzyme activities and gene expressions relative to the untreated control. The 1077 cultivar the resistant hybrid consistently outperformed Dania 85 in enzyme activities and gene expression.
PS and ABA significantly enhanced growth (≤ 57.4%↑) and defense (≤ 3.3-fold↑), with 1077 exhibiting superior resistance (48-53%↓ pathology). This hybrid is already marketed as a resistant cultivar, underscoring the potential for integrated disease management strategies.
评估了脱落酸（ABA）、多胺（PS）等各种处理以及哈茨木霉生物防治与南方根结线虫之间的相互作用，以评估它们对番茄品种病理指标、生长指标和防御机制的影响。
该研究评估了两个番茄品种（1077和达尼亚85）中南方根结线虫对番茄根系的病害（虫瘿和卵块数量）以及生长参数（地上部、根系和果实重量），同时还评估了防御酶的活性；过氧化物酶（POD）、多酚氧化酶（PPO）以及防御基因（POD、PPO和PR2）的表达。具体而言，该研究在接种后45天的温室条件下评估了脱落酸（ABA；10μM）、硅酸钾（5g/L）和哈茨木霉生物制剂（10CFU/ml）处理。测量了包括虫瘿和卵块数量在内的病理指标，以及防御酶活性和基因表达水平。
在所应用的处理中，1077品种表现出最高的抗性，虫瘿和卵块数量最少。与未处理的线虫感染对照相比，所有处理（ABA、PS和木霉）均显著提高了生长参数。具体而言，PS处理在两个品种（1077和达尼亚85）的地上部鲜重方面表现出最强的促进作用。所有处理中PPO和POD的酶活性以及POD、PPO和PR2的基因表达均有所升高。PS是最有效的处理方法，其次是ABA和木霉，相对于未处理对照，显著提高了酶活性和基因表达。抗性杂交品种1077在酶活性和基因表达方面始终优于达尼亚85。
PS和ABA显著促进了生长（最多提高57.4%）和防御（最多提高3.3倍），1077表现出更高的抗性（病理降低48 - 53%）。该杂交品种已作为抗性品种上市，突出了综合病害管理策略的潜力。
