[LINC 00478在口腔鳞状细胞癌中的表达及其对增殖、迁移和侵袭的影响]

[Expression of LINC 00478 in oral squamous cell carcinoma and its effect on proliferation, migration and invasion].

作者信息

Zhao Qian, Han Linzi, Zhao Lu, Wang Lili, Zhuang Likun, Yuan Rongtao

机构信息

School of Stomatology, Qingdao University. Qingdao 266003, China. E-mail:

出版信息

Shanghai Kou Qiang Yi Xue. 2025 Apr;34(2):126-131.

PMID:40550762

Abstract

PUEPOSE

To examine the expression difference of LINC 00478 in oral squamous cell carcinoma (OSCC) and analyze its relationship with the prognosis of OSCC patients. To investigate the effects of LINC 00478 on proliferation, migration, and invasion of OSCC cells, with the goal of offering a new potential approach for molecular detection and clinical diagnosis of OSCC.

METHODS

Transcriptome data samples from OSCC were obtained from TCGA, and R software was utilized to assess the expression variance of LINC 00478 in OSCC tumor and normal tissues. Tissue samples from 26 OSCC patients, including cancer and adjacent tissues, were collected for RT-PCR analysis to determine the differential expression of LINC 00478 in OSCC tissues. In vitro experiments were conducted to explore the impact of LINC 00478 on proliferation, invasion, and migration of CAL27 cells. CCK-8 assay was used to observe the proliferation capability of LINC 00478 in CAL27 cells, while Transwell experiment was employed to assess its effects on cell migration and invasion. GraphPad Prism 8.0.2 software was used for statistical analysis of the data.

RESULTS

Analysis of the TCGA database revealed that LINC 00478 was weakly expressed in OSCC tissues compared to adjacent normal tissues (P＜0.001), with high-expression LINC 00478 correlating with improved patient prognosis(P＜0.05). Furthermore, LINC 00478 was significantly under-expressed in 26 OSCC tissues (P＜0.001). Experimental data from CCK-8 assays indicated that upregulation of LINC 00478 suppressed CAL27 proliferation (P＜0.05). Additionally, Transwell experiments demonstrated that high-expression LINC 00478 significantly inhibited CAL27 migration and invasion (P＜0.05).

CONCLUSIONS

LINC 00478 is significantly under-expressed in OSCC, and its high expression is positively correlated with a better prognosis. Over-expression of the LINC 00478 gene has been shown to inhibit the proliferation, migration, and invasion of CAL27 cells, suggesting a potential role in inhibiting cancer progression in OSCC.

摘要

目的

检测LINC 00478在口腔鳞状细胞癌（OSCC）中的表达差异，分析其与OSCC患者预后的关系。研究LINC 00478对OSCC细胞增殖、迁移和侵袭的影响，旨在为OSCC的分子检测和临床诊断提供一种新的潜在方法。

方法

从TCGA获取OSCC的转录组数据样本，利用R软件评估LINC 00478在OSCC肿瘤组织和正常组织中的表达差异。收集26例OSCC患者的组织样本（包括癌组织和癌旁组织）进行RT-PCR分析，以确定LINC 00478在OSCC组织中的差异表达。进行体外实验，探讨LINC 00478对CAL27细胞增殖、侵袭和迁移的影响。采用CCK-8法观察LINC 00478对CAL27细胞增殖能力的影响，采用Transwell实验评估其对细胞迁移和侵袭的作用。使用GraphPad Prism 8.0.2软件对数据进行统计分析。

结果

对TCGA数据库的分析显示，与相邻正常组织相比，LINC 00478在OSCC组织中表达较弱（P＜0.001），LINC 00478高表达与患者预后改善相关（P＜0.05）。此外，LINC 00478在26例OSCC组织中显著低表达（P＜0.001）。CCK-8实验数据表明，LINC 00478上调抑制了CAL27细胞的增殖（P＜0.05）。此外，Transwell实验表明，LINC 00478高表达显著抑制了CAL27细胞的迁移和侵袭（P＜0.05）。