Wang Shu-Zhen, Zhao Jun-Ran, Dong Yu-Mei, Zhang Wei, Ying Yue, Shu Jin-Ping, Zhang Jin-Ming, Zhang Peng-Jun
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 311121, China.
Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310018, China.
Sci Data. 2025 Jun 23;12(1):1070. doi: 10.1038/s41597-025-05411-z.
The leaf roller, Eumorphobotys obscuralis, is a highly destructive pest that causes severe defoliation, ultimately reducing plant vigor, leading to culm death, and resulting in significant economic and ecological losses. Despite its substantial impact, effective prevention and control strategies remain lacking, further exacerbating the damage caused by this species. To address the critical gap in genomic resources, we generated a high-quality chromosome-scale genome assembly for E. obscuralis using a combination of Illumina short reads, PacBio HiFi long reads, and Hi-C chromatin interaction data. The final assembly spans 701.9 Mb across 31 chromosomes, including the single sex chromosome Z. Key assembly metrics include a contig N50 of 1.73 Mb and a scaffold N50 of 23.70 Mb, with an estimated genome completeness of 96.7%. Genome annotation identified 15,855 protein-coding genes and revealed that repetitive sequences account for 58.9% of the genome. This genomic resource lays a solid foundation for exploring the genetic mechanisms underlying the pest's biology and adaptation, and serves as a valuable reference for the development of targeted pest management strategies.
卷叶蛾，Eumorphobotys obscuralis，是一种极具破坏性的害虫，会导致严重的叶片脱落，最终降低植物活力，导致茎秆死亡，并造成重大的经济和生态损失。尽管其影响巨大，但仍缺乏有效的预防和控制策略，这进一步加剧了该物种造成的损害。为了填补基因组资源方面的关键空白，我们结合Illumina短读长、PacBio HiFi长读长和Hi-C染色质相互作用数据，为E. obscuralis生成了高质量的染色体水平基因组组装。最终组装跨越31条染色体，长度为701.9 Mb，包括单条性染色体Z。关键的组装指标包括重叠群N50为1.73 Mb，支架N50为23.70 Mb，估计基因组完整性为96.7%。基因组注释鉴定出15,855个蛋白质编码基因，并表明重复序列占基因组的58.9%。这种基因组资源为探索该害虫生物学和适应性的遗传机制奠定了坚实基础，并为制定针对性的害虫管理策略提供了有价值的参考。
