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联合多组学分析揭示甲基化和miRNA靶向在多囊卵巢综合征中的作用

Combined Multiomics Analysis Reveals the Role of Methylation and miRNA-Targeted in Polycystic Ovary Syndrome.

作者信息

Ding Yicen, Huang Lishi, He Mengju, Zhang Fei, Jiang Yuanjun, Kang Yani

机构信息

School of Biomedical Engineering, Bio-ID Center Shanghai Jiao Tong University Shanghai China.

Peace Maternity and Child Health Hospital School of Medicine, Shanghai Jiao Tong University Shanghai China.

出版信息

Reprod Med Biol. 2025 Jun 23;24(1):e12664. doi: 10.1002/rmb2.12664. eCollection 2025 Jan-Dec.

DOI:10.1002/rmb2.12664
PMID:40551992
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12183603/
Abstract

PURPOSE

Polycystic ovary syndrome (PCOS) is an endocrine syndrome that afflicts women of childbearing age, whose specific pathogenesis is unknown. Combined multiomics analysis on it is still lacking. The purpose of this study was to use combined multiomics analyses to learn about the development of PCOS.

METHODS

We randomly selected three PCOS mouse models and two control mice as the mouse group, as well as three PCOS patients and two normal women as the human group. The data were analyzed by multiomics analysis including methylomes, transcriptomics, and metabolomics. We explored the key genes involved in the occurrence and development of PCOS that were common in multiomics. Methylation-specific polymerase chain reaction (MSP) and real-time PCR (qPCR) experiments were performed to verify the reliability of the results.

RESULTS

The gene was hypomethylated and highly expressed in both mouse and human samples. Meanwhile, had a lower expression in both mouse and human samples, targeted by an upregulated microRNA (miRNA) called has-miR-106a-5p, which may relate to hyperandrogenemia.

CONCLUSIONS

Epigenetic mechanisms have an impact on the development of PCOS. Both and play important roles in the pathological process of PCOS and have the potential to be diagnostic markers and therapeutic targets.

摘要

目的

多囊卵巢综合征（PCOS）是一种困扰育龄女性的内分泌综合征，其具体发病机制尚不清楚。目前仍缺乏对其进行多组学联合分析的研究。本研究旨在通过多组学联合分析来了解PCOS的发病机制。

方法

我们随机选取三只PCOS小鼠模型和两只对照小鼠作为小鼠组，以及三名PCOS患者和两名正常女性作为人类组。通过甲基化组学、转录组学和代谢组学等多组学分析方法对数据进行分析。我们探索了在多组学中共同存在的、参与PCOS发生发展的关键基因。进行甲基化特异性聚合酶链反应（MSP）和实时定量聚合酶链反应（qPCR）实验以验证结果的可靠性。

结果

该基因在小鼠和人类样本中均发生低甲基化且高表达。同时，在小鼠和人类样本中均有较低表达，被一种上调的名为has-miR-106a-5p的微小RNA（miRNA）靶向，这可能与高雄激素血症有关。

结论

表观遗传机制对PCOS的发病机制有影响。该基因和该基因在PCOS的病理过程中均起重要作用，并有潜力成为诊断标志物和治疗靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d1f/12183603/c85b10ef40bd/RMB2-24-e12664-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d1f/12183603/60ac7e871100/RMB2-24-e12664-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d1f/12183603/56ec9a9a3663/RMB2-24-e12664-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d1f/12183603/6e353940cacd/RMB2-24-e12664-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d1f/12183603/f73e02240103/RMB2-24-e12664-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d1f/12183603/5eb1e1184cce/RMB2-24-e12664-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d1f/12183603/2d0ab11e3742/RMB2-24-e12664-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d1f/12183603/1c78786b4ad4/RMB2-24-e12664-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d1f/12183603/61eacec4cbc1/RMB2-24-e12664-g004.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d1f/12183603/60ac7e871100/RMB2-24-e12664-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d1f/12183603/56ec9a9a3663/RMB2-24-e12664-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d1f/12183603/6e353940cacd/RMB2-24-e12664-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d1f/12183603/f73e02240103/RMB2-24-e12664-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d1f/12183603/5eb1e1184cce/RMB2-24-e12664-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d1f/12183603/2d0ab11e3742/RMB2-24-e12664-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d1f/12183603/1c78786b4ad4/RMB2-24-e12664-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d1f/12183603/61eacec4cbc1/RMB2-24-e12664-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d1f/12183603/c85b10ef40bd/RMB2-24-e12664-g003.jpg

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本文引用的文献

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The Pathophysiological Mechanism and Clinical Treatment of Polycystic Ovary Syndrome: A Molecular and Cellular Review of the Literature.多囊卵巢综合征的病理生理学机制与临床治疗：文献的分子与细胞综述。
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