脑肿瘤干细胞的高效迁移分析

Efficient Migration Assay for Brain Tumor Stem Cells.

作者信息

Mobilio Daniel, Kieliszek Agata M, Venugopal Chitra, Singh Sheila K, Chafe Shawn C

机构信息

Centre for Discovery in Cancer Research, McMaster University, Hamilton, ON, Canada.

Department of Biochemistry and Biomedical Sciences, Hamilton, ON, Canada.

出版信息

Methods Mol Biol. 2025;2944:141-148. doi: 10.1007/978-1-0716-4654-0_11.

DOI:10.1007/978-1-0716-4654-0_11

PMID:40553280

Abstract

A defining characteristic of brain tumor stem cells (BTSCs) is their ability to migrate and invade the brain parenchyma. This invasive behavior is particularly critical in understanding how BTSCs originating from primary tumors contribute to the formation of secondary brain tumor lesions. In this chapter, we present a streamlined and unbiased method to assess BTSC migration capacity. By leveraging real-time imaging with the Incucyte system, we provide a standardized and efficient approach for studying BTSC motility under controlled conditions.

摘要

脑肿瘤干细胞（BTSCs）的一个决定性特征是它们迁移和侵袭脑实质的能力。这种侵袭行为对于理解源自原发性肿瘤的BTSCs如何促成继发性脑肿瘤病变的形成尤为关键。在本章中，我们介绍一种简化且无偏差的方法来评估BTSC的迁移能力。通过利用Incucyte系统进行实时成像，我们提供了一种在可控条件下研究BTSC运动性的标准化高效方法。