脑肿瘤干细胞的分离与增殖

Isolation and Propagation of Brain Tumor Stem Cells.

作者信息

Puri Anish, Venugopal Chitra, Singh Sheila K

机构信息

Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON, Canada.

Centre for Discovery in Cancer Research (CDCR), McMaster University, Hamilton, ON, Canada.

出版信息

Methods Mol Biol. 2025;2944:17-25. doi: 10.1007/978-1-0716-4654-0_2.

DOI:10.1007/978-1-0716-4654-0_2

PMID:40553271

Abstract

Shortly after the discovery of cancer stem cells in acute myeloid leukemia, cancer stem cells in several cancer types were discovered, including brain cancer. Brain tumor stem cells (BTSCs), initially characterized by the expression of CD133, are a population of cells that can proliferate and form spheres in vitro and form tumors in vivo. Due to the belief that they are treatment-resistant and seed-recurrent diseases, they are an ideal preclinical model that can be used to study brain cancer. In this chapter, we describe how to process, maintain, and propagate a BTSC culture from brain tumor tissue samples.

摘要

在急性髓系白血病中发现癌症干细胞后不久，在包括脑癌在内的几种癌症类型中也发现了癌症干细胞。脑肿瘤干细胞（BTSCs）最初以CD133的表达为特征，是一群能够在体外增殖并形成球体、在体内形成肿瘤的细胞。由于人们认为它们具有抗治疗性并引发复发性疾病，所以它们是一种可用于研究脑癌的理想临床前模型。在本章中，我们描述了如何从脑肿瘤组织样本中处理、维持和传代培养BTSC。