Chimeric Antigen Receptor (CAR) T cells are T cells that have been engineered to specifically recognize antigens of interest on target cells, may that be in cancer or other pathologies. To date, CAR T cell therapy has solely been approved for use in liquid cancers, with many groups and trial working on CAR T cells for solid tumors. Herein, we describe the outline CAR T cell production using human peripheral blood mononuclear cells (PBMCs) and third-generation packaging vectors for use in in vitro and in vivo experimental assays.