Sultana Sumreen, Shwetha Hulikere Jagdish, Jayakumar Manju Nidagodu, Lakshmeesha Thimappa Ramachandrappa, Raichur Ashok M, Ravikiran Tekupalli
Department of Microbiology and Biotechnology, Bangalore University, Jnana Bharathi Campus, Bengaluru, India.
Department of Materials Engineering, Indian Institute of Science, Bengaluru, India.
Int J Biol Macromol. 2025 Aug;319(Pt 1):145359. doi: 10.1016/j.ijbiomac.2025.145359. Epub 2025 Jun 18.
The tuberous roots of Decalepis hamiltonii (D. hamiltonii) are a rich source of phenolic acids and flavonoids. Nanoencapsulation offers better drug delivery and enhances the bioavailability and stability of the bioactive compounds. In the current study, the cytotoxic ability of D. hamiltonii root extract-encapsulated chitosan nanoparticles (DHRE CS NPs) was investigated against HeLa cells. DLS analysis of DHRE CS NPs revealed the mean particle size (PS-245.4 ± 46.95 nm) and zeta potential (ZP-22.1 ± 13.7 mV). XRD and FTIR confirmed the amorphous nature and the successful encapsulation of DHRE. The SEM and HR-TEM analysis confirmed that the spherical-shaped particles are in the nano-range. The release studies revealed sustained release of around 96 % (pH 5.5) and 84 % (pH 7.4) up to 48 h. The MTT assay showed that DHRE CS NPs (IC-30 μg/mL) are more effective against HeLa cells. The cellular uptake studies revealed increased fluorescent intensity with time, indicating their efficient internalization. The DHRE CS NPs efficiently induced oxidative stress as confirmed by elevated ROS generation (72 %), reduced mitochondrial membrane potential (MMP-43 %), induction of apoptosis (58 %), and cell cycle arrest. Our study suggests that DHRE CS NPs could be a promising therapeutic strategy for cervical cancer.
汉密尔顿迪卡勒皮斯（Decalepis hamiltonii）的块根是酚酸和黄酮类化合物的丰富来源。纳米包封提供了更好的药物递送，并提高了生物活性化合物的生物利用度和稳定性。在本研究中，研究了汉密尔顿迪卡勒皮斯根提取物包封的壳聚糖纳米颗粒（DHRE CS NPs）对HeLa细胞的细胞毒性能力。对DHRE CS NPs的动态光散射分析揭示了平均粒径（PS - 245.4±46.95 nm）和zeta电位（ZP - 22.1±13.7 mV）。X射线衍射和傅里叶变换红外光谱证实了DHRE的无定形性质和成功包封。扫描电子显微镜和高分辨率透射电子显微镜分析证实球形颗粒处于纳米范围内。释放研究表明，在48小时内持续释放约96%（pH 5.5）和84%（pH 7.4）。MTT试验表明，DHRE CS NPs（IC - 30μg/mL）对HeLa细胞更有效。细胞摄取研究表明荧光强度随时间增加，表明它们有效地内化。如升高的活性氧生成（72%）、降低的线粒体膜电位（MMP - 43%）、凋亡诱导（58%）和细胞周期停滞所证实，DHRE CS NPs有效地诱导了氧化应激。我们的研究表明，DHRE CS NPs可能是一种有前途的宫颈癌治疗策略。
