TREM-1 as a potential biomarker for malignant transformation risk in oral leukoplakia: Insights from bioinformatics and dual immunofluorescence analysis.

BACKGROUND

Oral leukoplakia (OLK) is a precancerous lesion of oral squamous cell carcinoma (OSCC), but its malignant transformation mechanisms remain unclear. This study investigates TREM-1's role in OLK progression through bioinformatics and pathological validation, highlighting its potential as a prognostic biomarker for malignant transformation METHODS: TREM-1 expression in OLK was analyzed using Kaplan-Meier and Cox regression based on public datasets identified it as an independent risk factor Cox regression based on GEO clinical data. Single-cell RNA sequencing identified macrophages as the primary TREM-1-expressing cells. Dual-label immunofluorescence was performed on 69 OLK and 63 control tissues to validate TREM-1 expression and its co-localization with CD68. Statistical analysis included non-parametric tests, chi-square tests, and multivariate Cox regression to assess associations with malignant transformation. Significance was defined as p < 0.05.

RESULTS

Kaplan-Meier analysis showed that high TREM-1 expression was significantly associated with OLK malignant transformation (p = 0.007), and Cox regression based on public datasets identified it as an independent risk factor (HR = 2.702, p = 0.032). Single-cell RNA sequencing revealed macrophages as the main TREM-1-expressing cells. Immunofluorescence confirmed elevated TREM-1 expression in OLK macrophages compared to controls. In 69 clinical samples, chi-square analysis supported a significant association with malignant transformation, though this was not confirmed by Cox regression.

CONCLUSIONS

This study suggests that TREM-1 may be involved in OLK malignant transformation through macrophages. Although its expression is not related to dysplasia severity, it could serve as a biomarker for assessing the cancerous transformation.