Zhao Junmei, Liang Yaqian, Luo Qing
High-tech Hospital of the First Affiliated Hospital of Nanchang University，Nanchang，330006，China.
Department of Otorhinolaryngology-Head and Neck Surgery，the First affiliated Hospital of Nanchang University.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2025 Jul;39(7):624-631. doi: 10.13201/j.issn.2096-7993.2025.07.005.
The mucosa of Chronic rhinosinusitis with nasal polyps（CRSwNP） is accompanied by tissue remodeling. Epithelial-mesenchymal transition（EMT） plays an important role in tissue remodeling, but the mechanism of EMT is not yet clear. The purpose of this study is to further clarify the pathogenesis of CRSwNP and provide another idea and theoretical basis for the treatment of CRSwNP. ①The expression of NLRP3 and EMT-related protein（E-cadherin, Vimentin） in the nasal mucosa of the CRSwNP group and the normal control group were detected by immunohistochemistry（IHC）. ②Primary human nasal epithelial cells（HNECs） were cultured in vitro, and HNE-intervened cells with different concentrations（0, 10, 25, 50, 100 ng/mL） were used. After stimulation for 24 h, mRNA and protein expressions of E-cadherin, Vimentin, NLRP3 were detected by qRT-PCR and western blotting. ③Cells were collected at 0, 24, 36, 48 and 72 hours later after incubation with HNE with the optimal concentration, and the mRNA and protein expressions of E-cadherin, Vimentin and NLRP3 were detected by qRT-PCR and western blotting. ④Primary human nasal epithelial cells were pretreated with NLRP3 inhibitor MCC950, then stimulated with HNE, and EMT-related proteins（E-cadherin, Vimentin） and NLRP3 expression were detected by qRT-PCR and western blotting. ①The expression levels of NLRP3 and Vimentin in nasal polyps of CRSwNP patients were higher than those of control group, and the expression of E-cadherin was lower（<0.05）. The mRNA and protein expression levels of NLRP3 and Vimentin increased when HNE stimulated primary human nasal epithelial cells, while the expression of E-cadherin decreased. ②The effect was most significant when the HNE stimulated nasal mucosal epithelial cells were exposed to 50 ng/mL（<0.05）. The primary human nasal epithelial cells were stimulated with 50 ng/ml HNE, and the effect was most significant when the duration of HNE exposure was 36 h（<0.05）. ③Primary human nasal epithelial cells were pretreated with MCC950 and then stimulated with HNE. The mRNA and protein expression levels of E-cadherin in the NLRP3 inhibitor pretreated group were increased, while the mRNA and protein expression levels of Vimentin and NLRP3 were decreased（<0.05）. ln CRSwNP, HNE promotes EMT in human nasal mucosal epithelial cells by activating NLRP3.
伴鼻息肉的慢性鼻-鼻窦炎（CRSwNP）的黏膜伴有组织重塑。上皮-间质转化（EMT）在组织重塑中起重要作用，但EMT的机制尚不清楚。本研究的目的是进一步阐明CRSwNP的发病机制，并为CRSwNP的治疗提供新的思路和理论依据。①采用免疫组织化学（IHC）检测CRSwNP组和正常对照组鼻黏膜中NLRP3和EMT相关蛋白（E-钙黏蛋白、波形蛋白）的表达。②体外培养原代人鼻上皮细胞（HNECs），使用不同浓度（0、10、25、50、100 ng/mL）的HNE干预细胞。刺激24小时后，通过qRT-PCR和蛋白质印迹法检测E-钙黏蛋白、波形蛋白、NLRP3的mRNA和蛋白表达。③用最佳浓度的HNE孵育细胞后，分别在0、24、36、48和72小时收集细胞，通过qRT-PCR和蛋白质印迹法检测E-钙黏蛋白、波形蛋白和NLRP3的mRNA和蛋白表达。④原代人鼻上皮细胞用NLRP3抑制剂MCC950预处理，然后用HNE刺激，通过qRT-PCR和蛋白质印迹法检测EMT相关蛋白（E-钙黏蛋白、波形蛋白）和NLRP3的表达。①CRSwNP患者鼻息肉中NLRP3和波形蛋白的表达水平高于对照组，E-钙黏蛋白的表达较低（<0.05）。当HNE刺激原代人鼻上皮细胞时，NLRP3和波形蛋白的mRNA和蛋白表达水平升高，而E-钙黏蛋白的表达降低。②当HNE刺激鼻黏膜上皮细胞的浓度为50 ng/mL时，作用最显著（<0.05）。用50 ng/ml HNE刺激原代人鼻上皮细胞，当HNE暴露时间为36小时时，作用最显著（<0.05）。③原代人鼻上皮细胞用MCC950预处理后再用HNE刺激。NLRP3抑制剂预处理组中E-钙黏蛋白的mRNA和蛋白表达水平升高，而波形蛋白和NLRP3的mRNA和蛋白表达水平降低（<0.05）。在CRSwNP中，HNE通过激活NLRP3促进人鼻黏膜上皮细胞的EMT。
