Katagiri C, Kimura J, Murase N
J Biol Chem. 1985 Nov 5;260(25):13490-5.
The possible structure of lipophorin in insect blood (hemolymph) was investigated by differential scanning calorimetry (DSC) and 13C nuclear magnetic relaxation studies. The DSC heating curves of intact lipophorins showed endothermic peaks between -3 and 40 degrees C for lipophorins which contain hydrocarbons, whereas no such peaks were observed for lipophorins which do not contain this lipid. Hydrocarbon fractions isolated from the lipophorins showed endothermic peaks similar to those obtained from intact lipophorin in terms of the transition temperatures, the shapes, and the enthalpy changes. 13C spin lattice relaxation times of the (CH2)n resonance of hydrocarbons of intact lipophorin were measured as a function of temperature and revealed that the motions of hydrocarbon chains changed coincidentally with the onset and offset of phase transition. These data suggest the presence of a hydrocarbon-rich region within the lipophorin particles.
通过差示扫描量热法（DSC）和13C核磁共振弛豫研究，对昆虫血液（血淋巴）中脂转运蛋白的可能结构进行了研究。完整脂转运蛋白的DSC加热曲线显示，含有碳氢化合物的脂转运蛋白在-3至40摄氏度之间出现吸热峰，而不含这种脂质的脂转运蛋白则未观察到此类峰。从脂转运蛋白中分离出的碳氢化合物组分在转变温度、形状和焓变方面显示出与从完整脂转运蛋白中获得的相似的吸热峰。完整脂转运蛋白碳氢化合物的(CH2)n共振的13C自旋晶格弛豫时间作为温度的函数进行了测量，结果表明碳氢化合物链的运动与相变的开始和结束同时发生变化。这些数据表明脂转运蛋白颗粒内存在一个富含碳氢化合物的区域。
