Luo Wanhe, Jiang Yongtao, Liu Jinhuan, Algharib Samah Attia, Dawood Ali Sobhy, Xie Shuyu
Engineering Laboratory for Tarim Animal Diseases Diagnosis and Control, College of Animal Science and Technology, Tarim University, Alar 843300, China.
College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
Gels. 2025 May 22;11(6):380. doi: 10.3390/gels11060380.
The hyperglycemic microenvironment in diabetic wounds predisposes them to bacterial infections, sustains chronic inflammation, and hinders therapeutic efficacy. In this study, antibiotic-loaded fast-crosslinked hybrid nanogel wound dressings (florfenicol nanogels) based on Schiff's base bond were obtained through N, O-carboxymethyl chitosan (N, O-CMCS) and oxidized hyaluronic acid (OHA). The successfully prepared florfenicol N, O-CMCS/OHA nanogels exhibited obvious pH- and HAase-responsiveness release, which allowed it to quickly release florfenicol at infected wounds to exert on-demand antibacterial activity, as well as accelerate diabetic bacterial-infected wound healing. The nanogel dressings showed excellent antibacterial activity by destroying the bacterial cell membrane and wall. More specifically, the glucose oxidase in the dressings can catalyze the breakdown of high-concentration glucose, generating abundant ROS that directly cause cellular damage. According to the results of wound healing, the dressings showed satisfactory anti-inflammatory and therapeutic effects for the full-thickness mouse skin defect wounds. The nanogel dressings are anticipated to be excellent wound dressings to synergistically overcome the theraputic difficulty of diabetic bacterial wounds.
糖尿病伤口中的高血糖微环境使其易受细菌感染，维持慢性炎症，并阻碍治疗效果。在本研究中，通过N，O-羧甲基壳聚糖（N，O-CMCS）和氧化透明质酸（OHA）制备了基于席夫碱键的载抗生素快速交联混合纳米凝胶伤口敷料（氟苯尼考纳米凝胶）。成功制备的氟苯尼考N，O-CMCS/OHA纳米凝胶表现出明显的pH和透明质酸酶响应性释放，使其能够在感染伤口处快速释放氟苯尼考，发挥按需抗菌活性，并加速糖尿病细菌感染伤口的愈合。纳米凝胶敷料通过破坏细菌细胞膜和细胞壁表现出优异的抗菌活性。更具体地说，敷料中的葡萄糖氧化酶可以催化高浓度葡萄糖的分解，产生大量直接导致细胞损伤的活性氧。根据伤口愈合结果，该敷料对全层小鼠皮肤缺损伤口显示出令人满意的抗炎和治疗效果。预计纳米凝胶敷料将成为协同克服糖尿病细菌伤口治疗难题的优异伤口敷料。
