Cugnata Sarah, Sacheli Rosalie, Layios Nathalie, Hayette Marie-Pierre
Department of Clinical Microbiology, University Hospital of Liege, 4000 Liege, Belgium.
Department of Clinical Microbiology, Center for Interdisciplinary Research on Medicines (CIRM), University Hospital of Liege, 4000 Liege, Belgium.
J Fungi (Basel). 2025 Jun 19;11(6):465. doi: 10.3390/jof11060465.
Candidiasis is a major fungal infection worldwide, with invasive forms linked to high morbidity and mortality. The emergence of azole resistance in causing candidemia led us to examine the epidemiology and antifungal susceptibility of species at the University Hospital of Liège between January 2017 and December 2023. A total of 916 isolates from blood or sterile body fluids, tissues, and abscesses were analyzed. Species identification was performed using MALDI-TOF MS and antifungal susceptibility testing via Sensititre YO10 AST was interpreted according to the CLSI guidelines. remained the predominant species (56%), followed by (19%), (8%), and (7%). No significant shift toward non- species (NAC) was observed even during the COVID-19 pandemic, supporting the use of narrow-spectrum empirical therapy in selected patients. Fluconazole susceptibility was high in (98.8%), whereas and showed high resistance rates with 10.1% and 16.9%, respectively. showed stable fluconazole susceptibility across the study period. Echinocandins demonstrated excellent activity (95.6-100%), and amphotericin B was effective against nearly all isolates. This seven-year surveillance at the University Hospital of Liège confirms that while remains the predominant and highly susceptible species, rising azole resistance in non--particularly and -highlights the critical need for ongoing local epidemiological monitoring to guide effective and targeted antifungal therapy.
念珠菌病是一种全球范围内主要的真菌感染，侵袭性形式与高发病率和死亡率相关。引起念珠菌血症的唑类耐药性的出现促使我们在2017年1月至2023年12月期间，对列日大学医院的念珠菌属物种的流行病学和抗真菌药敏性进行研究。共分析了916株来自血液或无菌体液、组织及脓肿的分离株。使用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）进行菌种鉴定，并通过Sensititre YO10 AST进行抗真菌药敏试验，结果依据美国临床和实验室标准协会（CLSI）指南进行解读。白色念珠菌仍是主要菌种（56%），其次是光滑念珠菌（19%）、热带念珠菌（8%）和近平滑念珠菌（7%）。即使在新冠疫情期间，也未观察到向非白色念珠菌（NAC）的显著转变，这支持在特定患者中使用窄谱经验性治疗。白色念珠菌对氟康唑的敏感性较高（98.8%），而光滑念珠菌和热带念珠菌的耐药率较高，分别为10.1%和16.9%。近平滑念珠菌在整个研究期间对氟康唑的敏感性保持稳定。棘白菌素显示出优异的活性（95.6 - 100%），两性霉素B对几乎所有分离株均有效。列日大学医院的这七年监测证实，虽然白色念珠菌仍是主要且高度敏感的菌种，但非白色念珠菌尤其是光滑念珠菌和热带念珠菌的唑类耐药性不断上升，凸显了持续进行本地流行病学监测以指导有效且有针对性的抗真菌治疗的迫切需求。
