Liu Xiaojian, Ji Mingzhu, Zhang Jianzhen
Institute of Applied Biology, Shanxi University, Taiyuan 030006, China.
Shanxi Key Laboratory of Nucleic Acid Biopesticides, Shanxi University, Taiyuan 030006, China.
Insects. 2025 May 22;16(6):550. doi: 10.3390/insects16060550.
In eukaryotes, a lot of proteins are transported across the endoplasmic reticulum by the heterotrimeric Sec61 channel. And post-translational transport needs another Sec62/Sec63 complex. However, functions of these genes are poorly explored in insects. In this study, we first identified five Sec genes, named , , , and , in . Quantitative reverse-transcription polymerase chain reaction (RT-qPCR) analysis showed that these five genes were expressed in muti-tissues, including wing pad, leg, foregut, midgut, gastric cecum, hindgut, and highly expressed in the integument. Knockdown of and by RNA interference (RNAi) lead to the feeding cessation with a mortality rate of 100%. However, there is only 13.4% of ds-injected nymphs died before molting. All nymphs injected with ds and ds died before molting with the gut atrophy. Furthermore, hematoxylin-eosin staining indicated that the cells of the midguts and gastric caecum were defective, and the microvilli and peritrophic matrix were destroyed seriously after silencing and . Knockdown of and resulted in high mortality before and during molting. The hematoxylin-eosin (HE) staining and transmission electron microscopy (TEM) results showed that both the formation of the new cuticle and the degradation of the old cuticle were inhibited in ds-injected insects compared to the controls. Especially, there was no obvious plaques on microvillar tips of the epidermal cells after silencing of . These results revealed that and / genes are required in the gut and cuticle development of locusts. Therefore, these genes are potential targets for the control of locusts.
在真核生物中，许多蛋白质通过异源三聚体Sec61通道在内质网中运输。而翻译后转运则需要另一种Sec62/Sec63复合物。然而，这些基因在昆虫中的功能尚未得到充分研究。在本研究中，我们首先在[昆虫名称未给出]中鉴定出五个Sec基因，命名为[具体基因名称未给出]、[具体基因名称未给出]、[具体基因名称未给出]、[具体基因名称未给出]和[具体基因名称未给出]。定量逆转录聚合酶链反应（RT-qPCR）分析表明，这五个基因在多个组织中表达，包括翅芽、腿、前肠、中肠、胃盲囊、后肠，且在体壁中高表达。通过RNA干扰（RNAi）敲低[具体基因名称未给出]和[具体基因名称未给出]会导致取食停止，死亡率达100%。然而，注射双链RNA的若虫只有13.4%在蜕皮前死亡。所有注射ds[具体基因名称未给出]和ds[具体基因名称未给出]的若虫在蜕皮前死亡，肠道萎缩。此外，苏木精-伊红染色表明，沉默[具体基因名称未给出]和[具体基因名称未给出]后，中肠和胃盲囊的细胞出现缺陷，微绒毛和围食膜被严重破坏。敲低[具体基因名称未给出]和[具体基因名称未给出]导致在蜕皮前和蜕皮期间死亡率很高。苏木精-伊红（HE）染色和透射电子显微镜（TEM）结果表明，与对照相比，注射双链RNA的昆虫中新表皮的形成和旧表皮的降解均受到抑制。特别是，沉默[具体基因名称未给出]后，表皮细胞微绒毛尖端没有明显的斑块。这些结果表明，[具体基因名称未给出]和[具体基因名称未给出]/[具体基因名称未给出]基因在蝗虫肠道和表皮发育中是必需的。因此，这些基因是控制蝗虫的潜在靶点。
