Lambrechts M, Rasmussen K E
J Chromatogr. 1985 Sep 6;331(2):339-48. doi: 10.1016/0021-9673(85)80040-6.
A simple and rapid method has been developed for the extraction of impurities from illicit amphetamine samples using bonded-phase silica sorbents. The drug is dissolved in phosphate buffer (pH 7) and added to a C8 Bond Elut extraction column. The column is washed with water, and the impurities are then eluted with acetonitrile. The eluate is directly injected into the liquid chromatograph. This sample preparation technique has been compared with the traditional liquid-liquid extraction method. High-performance liquid chromatographic analysis of the impurities is carried out on a reversed-phase C18 column with an acetonitrile-water gradient as mobile phase. Peaks are monitored by UV detection at 220 and 254 nm. A series of seized amphetamine samples has been analysed, and the procedure gives detailed impurity patterns suitable for the comparison of samples. Compounds are identified by absorbance ratios (A220/A254).
已开发出一种简单快速的方法，用于使用键合相硅胶吸附剂从非法苯丙胺样品中提取杂质。将药物溶解在磷酸盐缓冲液（pH 7）中，然后加入到C8 Bond Elut萃取柱中。用水冲洗柱子，然后用乙腈洗脱杂质。洗脱液直接注入液相色谱仪。该样品制备技术已与传统的液 - 液萃取方法进行了比较。在以乙腈 - 水梯度为流动相的反相C18柱上对杂质进行高效液相色谱分析。通过在220和254 nm处的紫外检测监测峰。对一系列查获的苯丙胺样品进行了分析，该程序给出了适合样品比较的详细杂质图谱。通过吸光度比（A220/A254）鉴定化合物。
