通过在亲水性凝胶柱上进行等度洗脱分离抗坏血酸、脱氢抗坏血酸、二酮古洛糖酸和葡萄糖。

Separation of ascorbic acid, dehydroascorbic acid, diketogulonic acid and glucose by isocratic elution from a column of a hydrophilic gel.

作者信息

Seki T, Yamaguchi Y, Noguchi K, Yanagihara Y

出版信息

J Chromatogr. 1985 Sep 20;332:283-6. doi: 10.1016/s0021-9673(01)83308-2.

Abstract

High-performance liquid chromatography on an Asahipak GS-320 hydrophilic gel column with tartrate buffer (0.015 M, pH 3.0) containing 2 mM ethylenediaminetetraacetate and 0.05% beta-thiodiglycol as the eluent allowed the separation of glucose, diketogulonic acid, dehydroascorbic acid and ascorbic acid within 30 min. Fluorimetric monitoring of these compounds in the eluate with benzamidine at alkaline pH and at 90 degrees C in the presence of potassium sulphite allowed the determination of nanogram amounts of ascorbic acid, dehydroascorbic acid and diketogulonic acid. This method was applied to the determination of ascorbic acid in fruit juice.

摘要

采用Asahipak GS - 320亲水性凝胶柱进行高效液相色谱分析，以含有2 mM乙二胺四乙酸和0.05%β - 硫代二甘醇的酒石酸盐缓冲液（0.015 M，pH 3.0）作为洗脱剂，可在30分钟内分离葡萄糖、二酮古洛糖酸、脱氢抗坏血酸和抗坏血酸。在碱性pH值、90℃以及亚硫酸钾存在的条件下，用苯甲脒对洗脱液中的这些化合物进行荧光监测，能够测定纳克级含量的抗坏血酸、脱氢抗坏血酸和二酮古洛糖酸。该方法应用于果汁中抗坏血酸的测定。