Arunyanart M, Cline Love L J
J Chromatogr. 1985 Aug 9;342(2):293-301. doi: 10.1016/s0378-4347(00)84520-x.
Direct serum and urine injection, without sample extraction or protein precipitation steps, into a liquid chromatographic system using sodium dodecyl sulfate (SDS) with 10% added propanol as the mobile phase, is described for measurement of drug levels. The ability of SDS micelles to form soluble protein-SDS complexes, with no on-column precipitation, provides a simple, rapid method for routine determination of quinine, quinidine, propranolol, morphine and codeine at concentration levels found in serum and urine following administration of therapeutic doses. Absolute limits of detection ranged from 0.2 to 6 ng. Variation of the surfactants mobile phase concentration allows control of selectivity and analysis time, although a minimum concentration is required to prevent protein precipitation. Chromatographic efficiencies are improved by the addition of propanol to the micellar mobile phase, and sensitivities improved by use of fluorescence detection. The sensitivities are more than adequate for therapeutic drug monitoring of concentration ranges normally encountered in serum and urine.
本文描述了一种在使用十二烷基硫酸钠（SDS）并添加10%丙醇作为流动相的液相色谱系统中，直接将血清和尿液注入而无需样品提取或蛋白质沉淀步骤来测量药物水平的方法。SDS胶束能够形成可溶性蛋白质-SDS复合物且不会在柱上沉淀，这为常规测定治疗剂量给药后血清和尿液中发现的奎宁、奎尼丁、普萘洛尔、吗啡和可待因的浓度水平提供了一种简单、快速的方法。绝对检测限范围为0.2至6纳克。尽管需要最低浓度以防止蛋白质沉淀，但改变表面活性剂流动相浓度可控制选择性和分析时间。向胶束流动相中添加丙醇可提高色谱效率，使用荧光检测可提高灵敏度。这些灵敏度对于血清和尿液中通常遇到的浓度范围的治疗药物监测而言绰绰有余。
