Wełna Jana, Napiórkowska-Mastalerz Marta, Cyrankiewicz Michał, Bogiel Tomasz, Kwiecińska-Piróg Joanna
Department of Microbiology, Faculty of Pharmacy, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, 85-067 Bydgoszcz, Poland.
Department of Biophysics, Faculty of Pharmacy, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, 85-067 Bydgoszcz, Poland.
Pathogens. 2025 May 27;14(6):536. doi: 10.3390/pathogens14060536.
A biofilm is a group of bacterial cells in the polysaccharide matrix bonded to the surface (biotic or abiotic). Clinicians now realize that most infections are biofilm-related. Biofilm infections are often induced by more than one bacterial species. The aim of this study is to characterize a mixed biofilm composed of and strains. Forty-six isolates derived from chronic wound infections were cultivated to establish mature biofilms. The biofilm biomass and cell viability were measured by colorimetric assays. strains were tested for the presence of virulence and biofilm-related genes. The quorum sensing assay using the biosensor strain was also performed. A mixed biofilm of and was visualized using fluorescence microscopy. Four groups of and pairs, also visualized with fluorescence microscopy, were distinguished based on the biofilm biomass growth and metabolic activity loss. The gene observed among isolates was connected to the metabolic activity loss of the biofilm. Generally, the interactions between and species are not uniform. It is crucial to further research the interactions between microorganisms in biofilms. This may provide information on the mechanisms of biofilm formation in the complicated chronic wound environment.
生物膜是附着在表面（生物或非生物）的多糖基质中的一群细菌细胞。临床医生现在认识到大多数感染都与生物膜有关。生物膜感染通常由不止一种细菌引起。本研究的目的是表征由[具体菌株1]和[具体菌株2]菌株组成的混合生物膜。从慢性伤口感染中分离出46株菌株，培养以形成成熟的生物膜。通过比色法测定生物膜生物量和细胞活力。检测[具体菌株1]和[具体菌株2]菌株是否存在毒力基因和生物膜相关基因。还使用生物传感器菌株进行了群体感应测定。使用荧光显微镜观察了[具体菌株1]和[具体菌株2]的混合生物膜。根据生物膜生物量生长和代谢活性丧失，区分了四组[具体菌株1]和[具体菌株2]对，也通过荧光显微镜观察到。在[具体菌株1]分离株中观察到的[具体基因]与生物膜的代谢活性丧失有关。一般来说，[具体菌株1]和[具体菌株2]物种之间的相互作用并不一致。进一步研究生物膜中微生物之间的相互作用至关重要。这可能为复杂慢性伤口环境中生物膜形成的机制提供信息。
