Van Hassel Wannes Hugo R, Guillaume Benoît, Masquelier Julien
Organic Contaminants and Additives, 1050 Sciensano, Belgium.
Toxins (Basel). 2025 Jun 6;17(6):285. doi: 10.3390/toxins17060285.
Toxic cyanobacterial blooms are prevalent in surface waters. Depending on several conditions, these blooms produce cyanotoxins. Human exposure to these toxins can occur through multiple routes, including contaminated crops through irrigation with contaminated water. Analytical methods have been developed for cyanotoxin quantification to investigate these exposures. Yet, proper comparisons between different labs via proficiency tests or interlaboratory comparison tests, as well as method quality assurance, are impossible. Developing reference materials for cyanotoxins in plants would help resolve these problems. Therefore, a novel liquid hydroculture setup was optimized to grow and contaminate strawberries. During fruit ripening, these plants were exposed to growth medium contaminated with pure microcystin-LR or freeze-dried cyanobacterial biomass containing different microcystin congeners. Afterwards, fruits, greens, and roots were harvested. Validated UHPLC-MS/MS methods were used to quantify the microcystin congeners in the growth medium and the plants. Furthermore, both contamination conditions resulted in the accumulation of toxin(s) in the roots and the greens. Yet in the contamination models, no toxin(s) accumulated in the fruits. Therefore, this contamination approach, combined with strawberries as a berry plant model, is only suitable for reference material production for limited matrices. Our cultivation model to produce reference material could be evaluated for other berry producers.
有毒蓝藻水华在地表水中普遍存在。根据多种条件，这些水华会产生蓝藻毒素。人类接触这些毒素可通过多种途径发生，包括用受污染的水灌溉导致农作物受污染。已开发出用于蓝藻毒素定量分析的方法来调查这些接触情况。然而，通过能力验证测试或实验室间比对测试在不同实验室之间进行恰当比较以及方法质量保证是不可能的。开发植物中蓝藻毒素的标准物质将有助于解决这些问题。因此，一种新型的液体水培装置被优化用于种植和污染草莓。在果实成熟期间，这些植物被暴露于被纯微囊藻毒素-LR或含有不同微囊藻毒素同系物的冻干蓝藻生物质污染的生长培养基中。之后，收获果实、绿叶和根部。使用经过验证的超高效液相色谱-串联质谱法对生长培养基和植物中的微囊藻毒素同系物进行定量。此外，两种污染情况均导致毒素在根部和绿叶中积累。然而在污染模型中，果实中没有毒素积累。因此，这种污染方法与作为浆果植物模型的草莓相结合，仅适用于有限基质的标准物质生产。我们生产标准物质的栽培模型可供其他浆果生产者评估。
