Doyle W J, Ingraham A S, Fireman P
J Allergy Clin Immunol. 1985 Oct;76(4):551-6. doi: 10.1016/0091-6749(85)90774-2.
Eustachian tube (ET) function was evaluated bilaterally by use of the inflation-deflation and forced-response test before and after nasal challenge with various doses of histamine in five adult and 10 infant rhesus monkeys (Macaca mulatta). Of the 20 experiments conducted on the infant and juvenile monkeys, functional ET obstruction resulted in six ears after a dose of 0.5 mg of histamine, in 10 ears after a dose of 1 mg, and in four ears after a dose of 2 mg. In contrast, of the 10 experiments conducted on the adult monkeys, functional ET obstruction developed in two ears after a dose of 1 mg of histamine and in two ears after a dose of 4 mg. Six ears failed to respond at doses of 8 mg of histamine. The ET obstruction was characterized by increases in the values of the passive function parameters and an abolition of the ability to equilibrate applied middle ear pressures by muscular activity. ET obstruction was not observed in any ears after challenge with a saline control. The severity of the induced ET obstruction was lessened within 60 minutes after challenge. These data are similar to those reported for intranasal pollen challenges in sensitized rhesus monkeys and human volunteers and suggest that type I allergic reactions at the site of the nasopharyngeal mucosa disrupts the normal functioning of the ET by a mechanism potentially mediated by histamine release.
在5只成年和10只幼年恒河猴（猕猴）中，在不同剂量组胺鼻腔激发前后，通过使用充气 - 放气和强制反应试验对双侧咽鼓管（ET）功能进行评估。在对幼年和成年猴子进行的20次实验中，0.5毫克组胺剂量后，6只耳朵出现功能性ET阻塞；1毫克剂量后，10只耳朵出现阻塞；2毫克剂量后，4只耳朵出现阻塞。相比之下，在对成年猴子进行的10次实验中，1毫克组胺剂量后，2只耳朵出现功能性ET阻塞；4毫克剂量后，2只耳朵出现阻塞。8毫克组胺剂量时，6只耳朵无反应。ET阻塞的特征是被动功能参数值增加，并且通过肌肉活动平衡中耳压力的能力丧失。用生理盐水对照激发后，任何耳朵均未观察到ET阻塞。激发后60分钟内，诱导的ET阻塞严重程度减轻。这些数据与在致敏恒河猴和人类志愿者中鼻内花粉激发所报告的数据相似，表明鼻咽黏膜部位的I型过敏反应通过组胺释放可能介导的机制破坏了ET的正常功能。
