Giller Katrin, Schmid Dominique, Serbetci Idil, Meleán Manuel, Greve Sarah, von Meyenn Ferdinand, Bollwein Heinrich, Herrera Carolina
Department of Molecular Nutritional Science, University of Hohenheim, Garbenstrasse 30, 70599 Stuttgart, Germany.
Animal Nutrition, ETH Zurich, Universitaetstrasse 2, 8092 Zurich, Switzerland.
Antioxidants (Basel). 2025 May 23;14(6):621. doi: 10.3390/antiox14060621.
In vitro fertilization is used to produce embryos from high-genetic-merit cattle. However, these embryos often exhibit inferior quality than those derived in vivo, possibly due to increased oxidative stress. This study investigates whether adding antioxidant polyphenols (resveratrol (RV), chlorogenic acid (CA), ellagic acid (EA)) to the in vitro maturation (IVM) medium at 0.25, 0.5, and 1 μM could improve embryo development. Oxygen consumption and gene expression were evaluated at the blastocyst stage following treatment with 1 μM of each polyphenol. Embryo development (cleavage, blastocyst, and hatched blastocyst rates) and oxygen consumption were not significantly affected by polyphenols. However, RV significantly upregulated the mRNA expression of the antioxidant enzyme glutathione peroxidase 4 (GPX4), while GPX4 expression was significantly downregulated by EA. Expression of other gene markers related to antioxidant defense, apoptosis, development, and metabolism was not significantly affected. The results indicate that applying RV, CA, and EA during bovine oocyte IVM does not enhance in vitro embryo development at the tested concentrations. Given the opposing effects of RV and EA on the expression of GPX4, the effects of those polyphenols regarding the protection of embryos from oxidative stress and potential long-term effects on the offspring remain to be elucidated.
体外受精用于培育具有高遗传价值的牛胚胎。然而，这些胚胎的质量往往比体内产生的胚胎差，这可能是由于氧化应激增加所致。本研究调查了在体外成熟（IVM）培养基中添加0.25、0.5和1μM的抗氧化多酚（白藜芦醇（RV）、绿原酸（CA）、鞣花酸（EA））是否能改善胚胎发育。在用1μM的每种多酚处理后，在囊胚阶段评估氧气消耗和基因表达。多酚对胚胎发育（卵裂、囊胚和孵化囊胚率）和氧气消耗没有显著影响。然而，RV显著上调了抗氧化酶谷胱甘肽过氧化物酶4（GPX4）的mRNA表达，而EA则显著下调了GPX4的表达。其他与抗氧化防御、细胞凋亡、发育和代谢相关的基因标志物的表达没有受到显著影响。结果表明，在牛卵母细胞IVM过程中应用RV、CA和EA，在测试浓度下不会增强体外胚胎发育。鉴于RV和EA对GPX4表达的相反作用，这些多酚在保护胚胎免受氧化应激方面的作用以及对后代的潜在长期影响仍有待阐明。
