Ramouzis Ilias, Oikonomopoulou Leda, Nanas Ioannis, Stamperna Konstantina, Amiridis Georgios S, Dovolou Eleni
Department of Obstetrics & Reproduction, Faculty of Veterinary Science, University of Thessaly, 43100 Karditsa, Greece.
Laboratory of Reproduction, Department of Animal Sciences, University of Thessaly, 41334 Larissa, Greece.
Animals (Basel). 2025 Jun 15;15(12):1767. doi: 10.3390/ani15121767.
Ghrelin, the endogenous ligand of the growth hormone secretagogue receptor, is released pre-prandially and during periods of negative energy balance, exhibiting anti-fertility properties. In this study, twenty ewes were divided into two groups: a ghrelin-treated group receiving 1.25 μg/kg body weight (BW) of ghrelin per day via mini-pumps for 28 days and an untreated control group. Estrus was synchronized, superovulation was induced with FSH, and embryos and follicular fluid were collected six days post-estrus. Blood samples were taken to measure LH, progesterone, and anti-Müllerian hormone (AMH) concentrations. Results indicated that in treated animals, preovulatory LH surge was weaker, and progesterone levels were lower than in controls. Differences were observed in the superovulatory response and the number of collected embryos, both being higher in controls. While AMH levels did not differ between groups at the beginning of the experiment, they were lower in treated animals at the time of FSH administration. Treated ewes exhibited a reduced number of small follicles, and their follicular fluid contained lower AMH concentrations than the controls. These findings suggest that ghrelin plays a direct role in regulating LH secretion from the pituitary and in controlling ovarian follicle development, highlighting the strong interaction between nutrition and fertility.
胃饥饿素是生长激素促分泌素受体的内源性配体，在餐前和负能量平衡期间释放，具有抗生育特性。在本研究中，将20只母羊分为两组：胃饥饿素处理组，通过微型泵每天接受1.25μg/kg体重的胃饥饿素，持续28天；未处理的对照组。使发情同步，用促卵泡素诱导超排卵，并在发情后6天收集胚胎和卵泡液。采集血样以测量促黄体生成素、孕酮和抗苗勒管激素（AMH）浓度。结果表明，在处理过的动物中，排卵前促黄体生成素高峰较弱，孕酮水平低于对照组。在超排卵反应和收集到的胚胎数量方面观察到差异，两者在对照组中均较高。虽然在实验开始时两组之间的AMH水平没有差异，但在给予促卵泡素时，处理过的动物中的AMH水平较低。处理过的母羊小卵泡数量减少，其卵泡液中的AMH浓度低于对照组。这些发现表明，胃饥饿素在调节垂体促黄体生成素分泌和控制卵巢卵泡发育中起直接作用，突出了营养与生育之间的强烈相互作用。
