Bianco Piero R
Department of Pharmaceutical Sciences, College of Pharmacy, University of Nebraska Medical Center, Omaha, NE 68198-6025, USA.
Int J Mol Sci. 2025 Jun 6;26(12):5441. doi: 10.3390/ijms26125441.
The maintenance of genome stability requires the coordinated actions of multiple proteins and protein complexes. One critical family of proteins is the recombination mediators. Their role is to facilitate the formation of recombinase nucleoprotein filaments on single-stranded DNA (ssDNA). Filament formation can take place on post-replicative ssDNA gaps as well as on 3'-tailed duplexes resulting from helicase-nuclease processing. In prokaryotes, the RecF, O, and R proteins are widely distributed and mediate RecA loading as either the RecFOR or RecOR complexes, depending on the species being studied. In this review, I compare and contrast the available biochemical and structural information to provide insight into the mechanism of action of this critical family of mediators.
基因组稳定性的维持需要多种蛋白质和蛋白质复合物的协同作用。其中一类关键的蛋白质是重组介导因子。它们的作用是促进在单链DNA（ssDNA）上形成重组酶核蛋白丝。丝的形成可以发生在复制后的ssDNA缺口上，也可以发生在解旋酶-核酸酶加工产生的3'端双链体上。在原核生物中，RecF、O和R蛋白广泛分布，并作为RecFOR或RecOR复合物介导RecA的加载，这取决于所研究的物种。在这篇综述中，我比较并对比了现有的生化和结构信息，以深入了解这一关键介导因子家族的作用机制。
