Feng Sibin, Wu Zinian, Tian Chunyu, Yang Yanting, Gong Wenlong, Li Zhiyong
Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot 010010, China.
Key Laboratory of Grassland Resources and Utilization of Ministry of Agriculture, Hohhot 010010, China.
Genes (Basel). 2025 May 28;16(6):652. doi: 10.3390/genes16060652.
: is a high-quality perennial forage grass in the Poaceae family, with significant ecological and economic value. While its chloroplast genome has been sequenced, the mitochondrial genome of this species remains poorly understood due to the inherent complexity and frequent recombination of plant mitochondrial genomes. : We sequenced the complete mitochondrial genome of using both Illumina Novaseq6000 and Oxford Nanopore PromethION platforms. Subsequently, comprehensive bioinformatics analyses were performed, including genome assembly and annotation, repetitive sequence identification, codon usage analysis, RNA editing site prediction, the detection of chloroplast-derived sequences, and phylogenetic reconstruction. : The mitochondrial genome of was determined to be 515,056 bp in length, with a GC content of 44.34%, similar to other Poaceae species. This genome encodes 35 protein-coding genes, 22 tRNA genes, and 10 rRNA genes. Repetitive sequences account for 16.2% of the genome, totaling 83,528 bp, including 124 simple sequence repeats, 293 dispersed repeats, and 31 tandem repeats. A total of 460 RNA editing sites were identified, among which 430 were nonsynonymous. Additionally, 110 putative chloroplast-derived sequences were detected. A phylogenetic analysis based on mitochondrial genome data clarified the species' evolutionary position within Poaceae. : This study provides genetic resources for evolutionary research on and the communication of organelle genomes. Meanwhile, it also lays a solid foundation for the better development and utilization of the germplasm resources of .
[物种名称]是禾本科一种优质多年生牧草,具有重要的生态和经济价值。虽然其叶绿体基因组已被测序,但由于植物线粒体基因组固有的复杂性和频繁的重组,该物种的线粒体基因组仍知之甚少。
我们使用Illumina Novaseq6000和Oxford Nanopore PromethION平台对[物种名称]的完整线粒体基因组进行了测序。随后进行了全面的生物信息学分析,包括基因组组装和注释、重复序列鉴定、密码子使用分析、RNA编辑位点预测、叶绿体衍生序列检测以及系统发育重建。
[物种名称]的线粒体基因组长度为515,056 bp,GC含量为44.34%,与其他禾本科物种相似。该基因组编码35个蛋白质编码基因、22个tRNA基因和10个rRNA基因。重复序列占基因组的16.2%,总计83,528 bp,包括124个简单序列重复、293个分散重复和31个串联重复。共鉴定出460个RNA编辑位点,其中430个为非同义编辑位点。此外,还检测到110个推定的叶绿体衍生序列。基于线粒体基因组数据的系统发育分析明确了该物种在禾本科中的进化位置。
本研究为[物种名称]的进化研究和细胞器基因组交流提供了遗传资源。同时,也为[物种名称]种质资源的更好开发和利用奠定了坚实基础。
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