Chen Xiaofei, Wu Zinian, Yang Yanting, Tao Qibo, Na Na, Wan Wenya, Tian Chunyu, Gong Wenlong, Li Zhiyong
College of Grassland Science, Qingdao Agricultural University, Qingdao, China.
Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot, China.
Front Plant Sci. 2025 Mar 11;16:1555595. doi: 10.3389/fpls.2025.1555595. eCollection 2025.
is a perennial leguminous herb and serves as a high-quality forage, playing a key role in both grassland ecological restoration and the development of grazing livestock farming.
In this study, we successfully assembled the mitochondrial genome and investigated various related aspects, including genomic features, RNA editing sites, codon preference, gene transfer events, and phylogeny.
We found that the length of the mitochondrial genome is 401,301 bp, and its GC content is 45.15%. It consists of 53 genes, comprising 32 protein-coding genes, 3 ribosomal RNA genes, and 18 transfer RNA genes. A total of 146 scattered repeats, 8 tandem repeats, and 124 simple sequence repeats are present in the mitochondrial genome. A thorough examination of all protein-coding genes revealed 485 instances of RNA editing and 9579 codons. Additionally, 57 homologous fragments were identified in mitochondrial genome and chloroplast genomes, accounting for approximately 4.04% of the mitochondrial genome. Furthermore, a phylogenetic tree based on mitochondrial genome data from 33 species belonging to four Fabaceae subfamilies and two species from other families validated the evolutionary relationship of Lotus. These findings have significant implications for understanding the organization and evolution of the mitochondrial genome as well as for the identification of genetic markers. They also offer valuable perspectives relevant to devising strategies for molecular breeding and evolutionary categorization of legumes.
是一种多年生豆科草本植物，作为优质饲料，在草原生态恢复和放牧畜牧业发展中都发挥着关键作用。
在本研究中，我们成功组装了线粒体基因组，并研究了包括基因组特征、RNA编辑位点、密码子偏好、基因转移事件和系统发育等各个相关方面。
我们发现线粒体基因组长度为401,301 bp，GC含量为45.15%。它由53个基因组成，包括32个蛋白质编码基因、3个核糖体RNA基因和18个转移RNA基因。线粒体基因组中共有146个散在重复序列、8个串联重复序列和124个简单序列重复序列。对所有蛋白质编码基因的全面检查发现了485个RNA编辑实例和9579个密码子。此外，在线粒体基因组和叶绿体基因组中鉴定出57个同源片段，约占线粒体基因组的4.04%。此外，基于来自豆科四个亚科的33个物种和其他科的两个物种的线粒体基因组数据构建的系统发育树验证了百脉根属的进化关系。这些发现对于理解线粒体基因组的组织和进化以及遗传标记的鉴定具有重要意义。它们还为豆类分子育种策略的制定和进化分类提供了有价值的观点。
