Zhang Xiaojun, Liang Ying, Bai Hao, Huang Quanhua, Liu Dongming, Ma Guanglei, Liu Xiangrui
Department of Pharmacology, Department of Gastroenterology, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310058, China.
Innovation Center of Yangtze Delta, Zhejiang University, Jiaxing 314100, China.
Foods. 2025 Jun 9;14(12):2031. doi: 10.3390/foods14122031.
In this study, we developed novel porous starch (PS)/ (LS) microcapsules via in situ cross-linking with sodium trimetaphosphate (STMP), using (LJ), (LA), and (LGG) as representative strains. Scanning electron microscopy (SEM) revealed that the cross-linked microcapsules (designated as PS/LS-CL: PS/LJ-CL, PS/LA-CL, PS/LGG-CL) formed aggregated structures with denser microarchitecture compared to uncross-linked porous starch/ microcapsules (designated as PS/LS: PS/LJ, PS/LA, PS/LGG). The encapsulation efficiencies of PS/LJ-CL, PS/LA-CL, and PS/LGG-CL (79.56%, 78.49%, and 55.96%, respectively) significantly surpassed those of their uncross-linked counterparts (67.92%, 58.68%, and 47.71%, < 0.05). In addition, the cross-linked porous starch microcapsules improved the survival rate of during simulated gastrointestinal digestion and long-time storage. Importantly, the oral gavage of PS/LS-CL, PS/LA-CL, and PS/LGG-CL significantly increased the amount of . The colonization efficiency of all the tested in mice was detected by both gradient dilution plate counting and quantitative real-time PCR (qRT-PCR). These findings indicate the potential function of the in situ cross-linked porous starch microcapsules as a robust delivery system to enhance the colonization of probiotics in vivo.
在本研究中，我们以三聚磷酸钠（STMP）通过原位交联法制备了新型多孔淀粉（PS）/（LS）微胶囊，使用（LJ）、（LA）和（LGG）作为代表性菌株。扫描电子显微镜（SEM）显示，与未交联的多孔淀粉/微胶囊（称为PS/LS：PS/LJ、PS/LA、PS/LGG）相比，交联微胶囊（称为PS/LS-CL：PS/LJ-CL、PS/LA-CL、PS/LGG-CL）形成了具有更致密微结构的聚集结构。PS/LJ-CL、PS/LA-CL和PS/LGG-CL的包封效率（分别为79.56%、78.49%和55.96%）显著超过其未交联对应物（67.92%、58.68%和47.71%，<0.05）。此外，交联多孔淀粉微胶囊提高了在模拟胃肠消化和长期储存期间的存活率。重要的是，口服灌胃PS/LS-CL、PS/LA-CL和PS/LGG-CL显著增加了的数量。通过梯度稀释平板计数和定量实时PCR（qRT-PCR）检测了所有测试的在小鼠中的定殖效率。这些发现表明原位交联多孔淀粉微胶囊作为一种强大的递送系统在增强益生菌体内定殖方面的潜在功能。
