Esponda P, Bedford J M
J Exp Zool. 1985 Jun;234(3):441-9. doi: 10.1002/jez.1402340311.
Fluid secreted by the rooster Wolffian duct contains several proteins separable on polyacrylamide gel electrophoresis (PAGE) and isoelectric focusing (IEF) gels. Antibodies against these fluid components were obtained by immunizing rabbits, and the IgG fraction was then purified. As judged by indirect immunofluorescence, purified IgG against rooster duct fluid did not bind to any testicular spermatozoa. However, it bound distinctly to the whole surface of spermatozoa from the initial (epididymal) region and more intensely to all spermatozoa from the mid- and terminal regions of the Wolffian duct of the rooster, though not at all to mature duck or pigeon spermatozoa. Thus, in the rooster, as in therian mammals, the surface of the spermatozoon clearly acquires specific components secreted by the Wolffian duct. It should not be assumed that such surface change in rooster spermatozoa is entirely comparable, in a functional sense, to that undergone by mammalian spermatozoa, in which this seems directly related to fertilizing ability. Unlike those of mammals, rooster spermatozoa do not seem to require capacitation, and some spermatozoa in the testis already are competent to fertilize. Components acquired in the Wolffian duct by the rooster spermatozoon may bear on other aspects, perhaps sperm transport and/or survival in the female.
公鸡沃尔夫管分泌的液体含有几种可通过聚丙烯酰胺凝胶电泳（PAGE）和等电聚焦（IEF）凝胶分离的蛋白质。通过免疫兔子获得针对这些液体成分的抗体，然后纯化IgG部分。通过间接免疫荧光判断，针对公鸡管液的纯化IgG不与任何睾丸精子结合。然而，它明显地结合到来自初始（附睾）区域的精子的整个表面，并且更强烈地结合到来自公鸡沃尔夫管中部和末端区域的所有精子，但完全不结合到成熟鸭或鸽的精子。因此，在公鸡中，与有胎盘哺乳动物一样，精子表面明显获得了沃尔夫管分泌的特定成分。不应假定公鸡精子的这种表面变化在功能意义上与哺乳动物精子所经历的完全可比，在哺乳动物中，这似乎与受精能力直接相关。与哺乳动物不同，公鸡精子似乎不需要获能，并且睾丸中的一些精子已经具有受精能力。公鸡精子在沃尔夫管中获得的成分可能与其他方面有关，也许与精子在雌性体内的运输和/或存活有关。
