Mohamadi Nasrabadi Ali, Eckstein Diana, Mettke Peter, Ghanem Nawras, Kallies René, Schmidt Matthias, Mothes Falk, Schaefer Thomas, Graefe Ricarda, Bandara Chaturanga D, Maier Melanie, Liebert Uwe Gerd, Richnow Hans, Herrmann Hartmut
Atmospheric Chemistry Department (ACD), Leibniz Institute for Tropospheric Research (TROPOS), Permoserstrasse 15, 04318 Leipzig, Germany.
Department of Technical Biogeochemistry, Helmholtz Centre for Environmental Research (UFZ), Permoserstrasse 15, 04318 Leipzig, Germany.
Environ Health (Wash). 2025 Mar 7;3(6):648-658. doi: 10.1021/envhealth.4c00215. eCollection 2025 Jun 20.
The COVID-19 pandemic highlighted the urgent need to control airborne virus transmission, particularly in indoor environments with limited ventilation. This study evaluates the effectiveness of UVA and UVC irradiation, along with hydrogen peroxide (HO), in inactivating aerosolized viruses. A 19 m virus aerosol simulation chamber, replicating indoor conditions, was used to simulate human respiratory emissions by aerosolizing phage T4 (T4 phages) embedded in a pig mucin medium that mimics respiratory aerosols. Results showed a clear, dose-dependent reduction in viral genome copies with UVC exposure, where a dose of 129.9 mJ/cm reduced over 99% of the viral genome copies. Although less efficient, UVA still contributed to virus inactivation, reducing detectable phages to 20% at 513.30 J/cm. Mucin provided a protective effect, making virus removal more challenging. Hydrogen peroxide enhanced disinfection, with 1.6 ppm reducing viral genome copies by 78%, and higher concentrations (up to 16 ppm) achieving over 99% reduction in the dark condition. The combination of UVA/UVC with HO further enhanced disinfection, eliminating detectable virus genome copies entirely. These findings underscore the potential for using combined UV light and chemical treatments to effectively mitigate airborne viral transmission in enclosed spaces.
新冠疫情凸显了控制空气传播病毒的紧迫性，尤其是在通风有限的室内环境中。本研究评估了紫外线A（UVA）和紫外线C（UVC）照射以及过氧化氢（HO）对雾化病毒的灭活效果。使用一个19立方米的病毒气溶胶模拟舱来模拟室内环境，通过雾化嵌入猪粘蛋白介质中的T4噬菌体（T4 phages）来模拟人类呼吸道排放，该介质可模拟呼吸气溶胶。结果表明，UVC照射后病毒基因组拷贝数呈现明显的剂量依赖性减少，剂量为129.9 mJ/cm²时可减少超过99%的病毒基因组拷贝数。虽然效率较低，但UVA仍有助于病毒灭活，在513.30 J/cm²时可将可检测到的噬菌体减少至20%。粘蛋白起到了保护作用，使病毒清除更具挑战性。过氧化氢可增强消毒效果，1.6 ppm可使病毒基因组拷贝数减少78%，更高浓度（高达16 ppm）在黑暗条件下可实现超过99%的减少。UVA/UVC与HO的组合进一步增强了消毒效果，可完全消除可检测到的病毒基因组拷贝数。这些发现强调了联合使用紫外线和化学处理来有效减轻封闭空间中空气传播病毒的可能性。
