Chai Jiale, Zhang Weiyi, Zhao Xue, Xu Xinglian
State Key Lab of Meat Quality Control and Cultured Meat Development, Ministry of Science and Technology, Key Laboratory of Meat Processing, Ministry of Agriculture, College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
State Key Lab of Meat Quality Control and Cultured Meat Development, Ministry of Science and Technology, Key Laboratory of Meat Processing, Ministry of Agriculture, College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
Food Chem. 2025 Nov 1;491:145227. doi: 10.1016/j.foodchem.2025.145227. Epub 2025 Jun 20.
Polysaccharides with higher charge density could improve the stability of myofibrillar protein (MP) aqueous solution. This study aimed to explore the molecular mechanism of stable MP mediated by carboxymethyl cellulose (CMC) with different degrees of substitution (DS = 0.7, 0.9, 1.2) through structural, rheological, and protein properties and molecular dynamics simulations. The results showed that the highest solubility of MP/CMC complex reached 72.99 % at the mass ratio of 40:1, which was an increase of 60.80 % compared to MP in the salt-free media. At molecular level, CMC with a higher DS stably affixed to the surface of MP molecules via strong electrostatic interactions and hydrogen bonds. Subsequently, the MP molecule conformations were rearranged forming the supramolecular ordering structure, which inhibited the self-assembly behavior. These results were confirmed in molecular docking and molecular dynamics simulations. This study provides valuable information for developing novel low-salt meat products based on the non-covalent modification of polysaccharides.
电荷密度较高的多糖可以提高肌原纤维蛋白（MP）水溶液的稳定性。本研究旨在通过结构、流变学、蛋白质性质和分子动力学模拟，探索不同取代度（DS = 0.7、0.9、1.2）的羧甲基纤维素（CMC）介导的MP稳定化的分子机制。结果表明，在质量比为40:1时，MP/CMC复合物的最高溶解度达到72.99%，与无盐介质中的MP相比增加了60.80%。在分子水平上，较高DS的CMC通过强静电相互作用和氢键稳定地附着在MP分子表面。随后，MP分子构象重新排列形成超分子有序结构，从而抑制了自组装行为。这些结果在分子对接和分子动力学模拟中得到了证实。本研究为基于多糖非共价修饰开发新型低盐肉制品提供了有价值的信息。
