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四川发酵中葡萄球菌噬菌体的分离、鉴定及初步应用

Isolation, Characterization, and Preliminary Application of Staphylococcal Bacteriophages in Sichuan Fermentation.

作者信息

Lin Xia, Deng Chunhui, Wang Luya, Shu Yue, Li Shengshuai, Song Yunlong, Kong Hong, Liang Ziwei, Liu Lei, Rao Yu

机构信息

Food Microbiology Key Laboratory of Sichuan Province, School of Food Science and Bioengineering, Xihua University, Chengdu 610039, China.

State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou 730000, China.

出版信息

Microorganisms. 2025 May 30;13(6):1273. doi: 10.3390/microorganisms13061273.

Abstract

Sichuan , a microbial food predominantly fermented by lactic acid bacteria and hosting a complex and diverse microbial ecosystem, serves as an ideal habitat for bacteriophages. However, relatively few studies have been conducted on isolating bacteriophages from fermented vegetables and their application in vegetable fermentation. In this study, three staphylococcal bacteriophages, ΦSx-2, ΦSs-1, and ΦSs-2, were isolated and purified from Sichuan using the spot test method. The morphological features of the phages were characterized using transmission electron microscopy (TEM), while key biological properties such as one-step growth kinetics were systematically evaluated, ultimately verifying their taxonomic placement within the Caudoviricetes class. Furthermore, the potential effects of these phages on the microbial community structure and physicochemical properties during fermentation were investigated using high-throughput sequencing and standard physicochemical assays. Microbial community analysis demonstrated that introducing the phages significantly increased the relative abundance of lactic acid bacteria while reducing the prevalence of spoilage bacteria such as , , and . Physicochemical assessments revealed that adding phages accelerated the acidification process of , effectively reduced nitrite levels, and increased the concentrations of lactic and acetic acids. Additionally, notable differences in color and flavor were observed between the two groups of during the fermentation process. In summary, the inoculation of bacteriophages ΦSx-2, ΦSs-1, and ΦSs-2 optimized the microbial community structure, enhanced the fermentation process, and improved the quality of Sichuan .

摘要

泡菜是一种主要由乳酸菌发酵的发酵食品,拥有复杂多样的微生物生态系统,是噬菌体的理想栖息地。然而,从发酵蔬菜中分离噬菌体及其在蔬菜发酵中的应用的研究相对较少。在本研究中,采用点滴试验法从泡菜中分离并纯化出三株葡萄球菌噬菌体,即ΦSx-2、ΦSs-1和ΦSs-2。利用透射电子显微镜(TEM)对噬菌体的形态特征进行了表征,同时系统评估了一步生长动力学等关键生物学特性,最终确定了它们在有尾噬菌体目(Caudoviricetes)中的分类地位。此外,利用高通量测序和标准理化分析方法,研究了这些噬菌体在发酵过程中对微生物群落结构和理化性质的潜在影响。微生物群落分析表明,引入噬菌体显著增加了乳酸菌的相对丰度,同时降低了腐败菌如[此处原文缺失相关腐败菌名称]的流行率。理化评估显示,添加噬菌体加速了泡菜的酸化过程,有效降低了亚硝酸盐水平,并增加了乳酸和乙酸的浓度。此外,在发酵过程中,两组泡菜在颜色和风味上也观察到了显著差异。总之,接种噬菌体ΦSx-2、ΦSs-1和ΦSs-2优化了微生物群落结构,增强了发酵过程,提高了泡菜的品质。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bab4/12195502/eb448c161367/microorganisms-13-01273-g001.jpg

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