Cardoso Olga, Donato Maria Manuel, Henriques Sara Carolina, Ramos Fernando
Chemical Engineering and Renewable Resources for Sustainability (CERES), Department of Chemical Engineering, Faculdade de Farmácia, Universidade de Coimbra, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal.
CIMAGO (Centro de Investigação em Meio Ambiente, Genética e Oncobiologia), Faculdade de Medicina, Universidade de Coimbra, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal.
Microorganisms. 2025 Jun 14;13(6):1389. doi: 10.3390/microorganisms13061389.
The presence of fluoroquinolone (FQ) residues in food-producing animals has raised concerns regarding antimicrobial resistance. This study evaluated the occurrence of FQ residues in the liver and kidneys of piglets and their association with resistance patterns in and species from piglets' intestinal microbiota. FQ residues were found in 44% of livers and 23% of kidneys. Among 340 isolates from feces, resistance to ciprofloxacin (CIP) (43.2%) and multidrug resistance (MDR) (82.7%) were prominent. The presence of FQ in kidneys significantly increased the odds of CIP-resistant (OR = 2.94, = 0.0013) and MDR (OR = 2.70, = 0.0047). Enterococci ( = 130) were evenly distributed among the species , , and spp. and presented resistance to CIP (34.6%). FQ in kidneys were associated with higher odds of CIP-resistant enterococci (OR = 3.78, = 0.015). Interaction models revealed species-dependent responses, with spp. showing high resistance in the presence of FQ in kidneys (OR = 18, = 0.006), while exhibited lower resistance compared to . These findings emphasize the role of FQ residues in promoting bacterial resistance and underscore the need for the stricter regulation and routine surveillance of antimicrobial use in livestock to curb the spread of bacterial resistance to clinical drugs, and mitigate public health risks-One Health.
食用动物体内氟喹诺酮（FQ）残留引发了人们对抗菌素耐药性的担忧。本研究评估了仔猪肝脏和肾脏中FQ残留的发生情况及其与仔猪肠道微生物群中 和 物种耐药模式的关联。在44%的肝脏和23%的肾脏中发现了FQ残留。在从粪便中分离出的340株 菌株中，对环丙沙星（CIP）的耐药率（43.2%）和多重耐药率（MDR）（82.7%）较为突出。肾脏中存在FQ显著增加了耐CIP （比值比=2.94， =0.0013）和MDR （比值比=2.70， =0.0047）的几率。肠球菌（ =130）在 、 和 属物种中分布均匀，对CIP的耐药率为34.6%。肾脏中的FQ与耐CIP肠球菌的较高几率相关（比值比=3.78， =0.015）。相互作用模型显示了物种依赖性反应， 属物种在肾脏中存在FQ时表现出高耐药性（比值比=18， =0.006），而 与 相比耐药性较低。这些发现强调了FQ残留在促进细菌耐药性方面的作用，并强调需要对牲畜抗菌药物的使用进行更严格的监管和常规监测，以遏制细菌耐药性向临床药物的传播，并降低公共卫生风险——一体化健康。
