Asgari Behnoush, Bradford Georgia, Hatje Eva, Kuballa Anna, Katouli Mohammad
Centre for Bioinnovation, and School of Science, Technology and Engineering, University of the Sunshine Coast, Maroochydore, QLD 4558, Australia.
Centre for Immunology and Infection Control, Faculty of Health, School of Biomedical Sciences, Queensland University of Technology, Brisbane, QLD 4000, Australia.
Microorganisms. 2025 Jun 19;13(6):1428. doi: 10.3390/microorganisms13061428.
Probiotics have been widely adopted due to their beneficial health properties. Here, we investigated the interactions of a probiotic M4-100, with a translocating strain HMLN-1, in a co-culture of cells, representing the intestinal epithelium, and identified molecular mechanisms associated with the host response. A co-culture of Caco-2:HT29-MTX cells was exposed to the HMLN-1 strain and the route of translocation was studied. Scanning and transmission electron microscopy revealed the adhesion of the strain to the microvilli, the establishment of close contact with the co-culture prior to being taken up by membrane-bound vesicles, and translocation via the intracellular pathway. When the HMLN-1 strain was challenged with M4-100 in co- and pre-inoculation experiments, its adhesion to the co-culture of cells was significantly reduced ( < 0.0001). A significant reduction in the invasion of the HMLN-1 strain was also observed upon the inoculation of M4-100 with the co-culture 60 min prior to HMLN-1 exposure ( < 0.0001). The M4-100 strain also significantly ( < 0.0001) reduced the translocation of the HMLN-1 strain in both co- and pre-inoculation experiments. Differential gene expression studies identified key cellular responses to the interaction with these bacteria, both alone. These data demonstrate the efficacy of M4-100 to reduce or inhibit the interaction of HMLN-1 with the intestinal epithelium. A prophylactic role of this probiotic strain is postulated as these effects were more pronounced in pre-inoculation experiments.
由于益生菌具有有益健康的特性，已被广泛应用。在此，我们研究了益生菌M4-100与易位菌株HMLN-1在代表肠上皮的细胞共培养体系中的相互作用，并确定了与宿主反应相关的分子机制。将Caco-2:HT29-MTX细胞共培养物暴露于HMLN-1菌株，并研究其易位途径。扫描电子显微镜和透射电子显微镜显示该菌株粘附于微绒毛，在被膜结合小泡摄取之前与共培养物建立紧密接触，并通过细胞内途径易位。在共接种和预接种实验中，当HMLN-1菌株与M4-100共同培养时，其对细胞共培养物的粘附显著降低（<0.0001）。在HMLN-1暴露前60分钟将M4-100与共培养物接种时，也观察到HMLN-1菌株的侵袭显著减少（<0.0001）。在共接种和预接种实验中，M4-100菌株也显著（<0.0001）降低了HMLN-1菌株的易位。差异基因表达研究确定了单独与这些细菌相互作用时关键的细胞反应。这些数据证明了M4-100减少或抑制HMLN-1与肠上皮相互作用的功效。由于这些作用在预接种实验中更为明显，推测该益生菌菌株具有预防作用。
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